Berman A E, Morozevich G E, Oborotova T A, Mazurov V I
Biokhimiia. 1987 Mar;52(3):493-502.
The biosynthesis and secretion of collagen proteins was studied in cultures of normal human embryo fibroblasts at different passages and growth stages as well as in cultures of human embryo fibroblasts transformed by oncogenic virus SV-40. It was found that normal fibroblasts maintain at a constant level the collagen synthesis throughout 20 passages, which is typical of proliferating and resting cells. Virus-transformed cells produce 3-4 times less collagen proteins on a per cell count. Normal and transformed fibroblasts do not differ in terms of total protein synthesis. Secretion of collagen and non-collagen proteins in transformed cell cultures appeared to be much lower than in normal cell cultures. Study of synthesized proteins by polyacrylamide gel electrophoresis showed that both types of cells secrete collagen proteins predominantly as polymers containing interchain S-S bonds of 3-helix molecules. Study of the protein-synthesizing activity of two polysomal fractions, i.e. membrane bound and free polysomes, isolated from the cells of both types in a cell-free system showed that membrane-bound polysomes from transformed fibroblasts synthesize collagen much less actively in comparison with normal cells. However, in transformed cells free polysomes, in contrast with normal cells, are active participants of a cell-free collagen protein synthesis.
在不同传代次数和生长阶段的正常人胚胎成纤维细胞培养物以及由致癌病毒SV - 40转化的人胚胎成纤维细胞培养物中，对胶原蛋白的生物合成和分泌进行了研究。结果发现，正常成纤维细胞在20代培养过程中胶原蛋白合成保持在恒定水平，这是增殖细胞和静止细胞的典型特征。按每个细胞计数，病毒转化细胞产生的胶原蛋白减少3 - 4倍。正常和转化的成纤维细胞在总蛋白合成方面没有差异。转化细胞培养物中胶原蛋白和非胶原蛋白的分泌明显低于正常细胞培养物。通过聚丙烯酰胺凝胶电泳对合成蛋白的研究表明，两种类型的细胞都主要以含有三螺旋分子链间S - S键的聚合物形式分泌胶原蛋白。对从两种类型细胞中分离出的两种多核糖体组分（即膜结合多核糖体和游离多核糖体）在无细胞系统中的蛋白质合成活性研究表明，与正常细胞相比，转化成纤维细胞的膜结合多核糖体合成胶原蛋白的活性要低得多。然而，与正常细胞不同，在转化细胞中游离多核糖体是无细胞胶原蛋白合成的活跃参与者。
