在注射了从大鼠小肠制备的mRNA的非洲爪蟾卵母细胞中诱导毒蕈碱型乙酰胆碱、5-羟色胺和P物质受体。
Induction of muscarinic acetylcholine, serotonin and substance P receptors in Xenopus oocytes injected with mRNA prepared from the small intestine of rats.
作者信息
Aoshima H, Iio H, Anan M, Ishii H, Kobayashi S
出版信息
Brain Res. 1987 Apr;388(1):15-20. doi: 10.1016/0169-328x(87)90016-7.
Serotonin and muscarinic acetylcholine (ACh) receptors were clearly induced in Xenopus oocyte injected with mRNA prepared from the small intestines of rats. Their response to ACh and serotonin was composed of 4 distinct components: fast and slow depolarization, slow hyperpolarization and large membrane potential fluctuation. About three-quarters of the injected oocytes responded to substance P. The response of the injected oocytes to substance P was transient and decayed even in the presence of substance P, indicating the presence of desensitization. However, the injected oocytes showed no response to 6 other drugs analyzed: adrenaline, noradrenaline, dopamine, gamma-aminobutyric acid, glycine and glutamate.
在用从大鼠小肠制备的mRNA注射的非洲爪蟾卵母细胞中，5-羟色胺和毒蕈碱型乙酰胆碱（ACh）受体被明显诱导。它们对ACh和5-羟色胺的反应由4个不同成分组成：快速和缓慢去极化、缓慢超极化和大的膜电位波动。约四分之三注射的卵母细胞对P物质有反应。注射的卵母细胞对P物质的反应是短暂的，即使在有P物质存在的情况下也会衰减，表明存在脱敏现象。然而，注射的卵母细胞对分析的其他6种药物无反应：肾上腺素、去甲肾上腺素、多巴胺、γ-氨基丁酸、甘氨酸和谷氨酸。
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