Department of Stomatology, Bogomolets National Medical University, 13, T. Shevchenko blvd, 01601, Kyiv, Ukraine.
János Szentágothai Research Centre & Department of Pharmacology and Pharmacotherapy, Medical School, University of Pecs, Ifjúság útja 20, Pécs, H-7624, Hungary.
J Craniomaxillofac Surg. 2018 Dec;46(12):2203-2213. doi: 10.1016/j.jcms.2018.09.022. Epub 2018 Sep 25.
The goal of this study was the evaluation of the bone tissue structural characteristics over the time course of mandibular defect healing using micro-CT technique, as well as determination of the inter-relationships between different micro-CT parameters used for assessment of the bone regeneration process and the patterns of their dynamic changes.
The body and ramus of the mandible was exposed in 24 Wistar rats. A 2-mm full thickness bony defect was created. Animals were randomized into four groups, which were ended 3, 6, 12 and 24 weeks after operation. The mandible was excised and underwent micro-CT analysis. For statistical evaluation, the Mann-Whitney U test, polynomial or exponential regression and Spearman analysis were applied.
The absolute volume of the bone regenerate increased from 1.69 ± 0.53 mm (3 weeks) to 3.36 mm ± 0.56 (6 months), as well as percentage of bone volume, increased significantly from 12.5 ± 2.3% at the 3-week term to 26.4 ± 8.7% at the 3-month term or 23.1 ± 8.7% at the 6-month term. Structural (trabecular) thickness gradually increased from 0.13 ± 0.007 mm at the 3-week term to 0.3 ± 0.11 mm at the 6-month term. The structural model index was 0.79 ± 0.46 in the early phase after trauma and then decreased to negative values.
The bone regeneration process was characterized by a significant increase (p < 0.05) in bone volume, percentage of bone volume, structural thickness and bone mineral density, and a decrease in bone surface-to-volume ratio and volume of pore space from the 3-week term to the 6-month term. These changes can be mathematically described by nonlinear exponential regression models.
本研究旨在通过 micro-CT 技术评估下颌骨缺损愈合过程中的骨组织结构特征，并确定用于评估骨再生过程的不同 micro-CT 参数之间的相互关系及其动态变化模式。
在 24 只 Wistar 大鼠中暴露下颌骨体和支。创建 2mm 全层骨缺损。动物随机分为四组，分别于术后 3、6、12 和 24 周结束。切除下颌骨并进行 micro-CT 分析。为了进行统计评估，应用了曼-惠特尼 U 检验、多项式或指数回归以及斯皮尔曼分析。
骨再生体的绝对体积从 1.69±0.53mm（3 周）增加到 3.36mm±0.56mm（6 个月），骨体积百分比也从 3 周时的 12.5±2.3%显著增加到 3 个月时的 26.4±8.7%或 6 个月时的 23.1±8.7%。结构（小梁）厚度从 3 周时的 0.13±0.007mm 逐渐增加到 6 个月时的 0.3±0.11mm。结构模型指数在创伤后早期为 0.79±0.46，然后降低至负值。
骨再生过程的特征是骨体积、骨体积百分比、结构厚度和骨矿物质密度显著增加（p<0.05），从 3 周时到 6 个月时骨表面体积比和孔隙空间体积减少。这些变化可以通过非线性指数回归模型进行数学描述。
