Imamura S, Armstrong E G, Birken S, Cole L A, Canfield R E
Clin Chim Acta. 1987 Mar 30;163(3):339-49. doi: 10.1016/0009-8981(87)90252-x.
Urinary forms of human chorionic gonadotropin (hCG) with oligosaccharides deficient in sialic acid content (ashCG) have been reported to be excreted by patients with choriocarcinoma in greater amounts than by healthy, pregnant women. Although ashCG potentially could be a useful marker for the diagnosis and management of gestational trophoblastic neoplasia, the methods previously used for its detection were not suitable for routine clinical application. Therefore, we have devised a simpler method which can provide specific and sensitive measurements of ashCG in urine. This method, which is designated as a lectin-immunoradiometric assay (LIRMA), employs an agarose-coupled lectin to selectively extract the ashCG, which is then quantified directly with a purified and radiolabelled rabbit antibody. The LIRMA has been applied to demonstrate that there is an increased excretion of ashCG by choriocarcinoma patients. It is also applicable, in principle, for the study of any glycoprotein which has a reduced content of sialic acid in its carbohydrate side chains.
据报道，患有绒毛膜癌的患者排泄的尿中形式的人绒毛膜促性腺激素（hCG）中唾液酸含量低的寡糖（去唾液酸hCG，ashCG）比健康孕妇更多。尽管ashCG可能是妊娠滋养细胞肿瘤诊断和管理的有用标志物，但以前用于检测它的方法不适用于常规临床应用。因此，我们设计了一种更简单的方法，该方法可以对尿液中的ashCG进行特异性和灵敏的测量。这种方法被称为凝集素免疫放射测定法（LIRMA），它使用琼脂糖偶联凝集素来选择性提取ashCG，然后用纯化的放射性标记兔抗体直接对其进行定量。LIRMA已被用于证明绒毛膜癌患者的ashCG排泄增加。原则上，它也适用于研究碳水化合物侧链中唾液酸含量降低的任何糖蛋白。
