Kaneda Y, Hayes H, Uchida T, Yoshida M C, Okada Y
Chromosoma. 1987;95(1):8-12. doi: 10.1007/BF00293835.
A human-mouse hybrid segregant HM76Dd40-6 with new characteristics was derived from the hybrid cell line HM76Dd containing human chromosome 19 as the only human chromosome. Three virus sensitivities located on human chromosome 19 (PVS, E11S and RDRC) were lost in HM76Dd40-6, while six other genes (C3, LDLR, EF2, GPI, PEPD and MANB) were retained. Cytogenetic analysis and in situ hybridization using human or mouse repeated sequences as probes showed that the region q13.1-qter of human chromosome 19 had been replaced by a fragment of mouse chromosome. Our results permit further regional assignment for the following five genes on human chromosome 19: GPI in the region cen-q12, MANB in p13.2-q12, E11S and RDRC in q13.1-qter, and EF2 in pter-q12.
一种具有新特性的人-鼠杂交分离株HM76Dd40-6源自杂交细胞系HM76Dd，该杂交细胞系含有人类19号染色体作为唯一的人类染色体。位于人类19号染色体上的三种病毒敏感性（PVS、E11S和RDRC）在HM76Dd40-6中丧失，而其他六个基因（C3、LDLR、EF2、GPI、PEPD和MANB）得以保留。细胞遗传学分析以及使用人类或小鼠重复序列作为探针的原位杂交表明，人类19号染色体的q13.1-qter区域已被小鼠染色体的一个片段所取代。我们的结果允许对人类19号染色体上的以下五个基因进行进一步的区域定位：cen-q12区域的GPI、p13.2-q12区域的MANB、q13.1-qter区域的E11S和RDRC以及pter-q12区域的EF2。
