Gulumian M, Kilroe-Smith T A
Environ Res. 1987 Jun;43(1):267-73. doi: 10.1016/s0013-9351(87)80077-4.
Lipid peroxidation is increased by ferrous or ferric ions in rat lung microsomes both from rats pretreated with 3-methylcholanthrene and from untreated controls. This increase was dependent on the concentration of these ions in the reaction mixture. Crocidolite alone increased peroxidation in microsomal fractions. However, addition of ferric or ferrous ions with the crocidolite did not give a greater increase in the amount of peroxidation in microsomes. Chelation with EDTA of iron, whether originally present as free ions in the solution or attached to crocidolite, prevented lipid peroxidation. NADPH alone, when added to the microsomal fractions, did not produce any significant effect. However, when added concomitantly with crocidolite fibers, NADPH reduced the effect of crocidolite on lipid peroxidation. Magnesium, manganese, and calcium ions did not produce any significant effect on lipid peroxidation in the presence or absence of crocidolite. A reduced pH enhanced the rate of lipid peroxidation in line with increased solubility of iron salts at these pH values. All the above observations, taken together, lead to the conclusion that it is the iron in the crocidolite that is responsible for the latter's ability to enhance lipid peroxidation.
在经3 - 甲基胆蒽预处理的大鼠及未处理的对照大鼠的肺微粒体中，亚铁离子或铁离子均可增加脂质过氧化作用。这种增加取决于反应混合物中这些离子的浓度。单独的青石棉可增加微粒体组分中的过氧化作用。然而，青石棉与铁离子一起添加时，并不会使微粒体中的过氧化作用量有更大增加。用乙二胺四乙酸（EDTA）螯合铁，无论铁最初是以溶液中的游离离子形式存在还是附着在青石棉上，均可防止脂质过氧化。单独将烟酰胺腺嘌呤二核苷酸磷酸（NADPH）添加到微粒体组分中时，不会产生任何显著影响。然而，当与青石棉纤维同时添加时，NADPH可降低青石棉对脂质过氧化的影响。在有或没有青石棉存在的情况下，镁离子、锰离子和钙离子对脂质过氧化均未产生任何显著影响。pH降低会提高脂质过氧化速率，这与铁盐在这些pH值下溶解度增加一致。综合上述所有观察结果，可得出结论：青石棉中的铁是其增强脂质过氧化能力的原因。
