School of Chemistry , Sun Yat-Sen University , Guangzhou 510275 , PR China.
School of Pharmacy , Guangdong Pharmaceutical University , Guangzhou 510006 , PR China.
Anal Chem. 2018 Nov 20;90(22):13373-13377. doi: 10.1021/acs.analchem.8b03052. Epub 2018 Oct 30.
Early diagnosis of HIV biomarkers or genes is the key to reducing acquired immunodeficiency syndrome (AIDS) mortality. In our work, we developed a novel polymerase chain reaction-dynamic light scattering (PCR-DLS) assay for one-step sensitive detection of HIV DNA based on the average-diameter change of gold nanoparticles (AuNPs). This is the first PCR assay that makes use of the DLS technique as a signal read-out, with the particle size measured by DLS increasing with the concentration of target DNA. With the help of the AuNP probes, this PCR-DLS assay can effectively improve the specificity of PCR reactions, which can greatly increase the detection sensitivity, with a detection limit of 1.8 aM (S/N = 3). In addition, the proposed strategy was successfully used to analyze target DNA in human serum samples, indicating that the PCR-DLS assay has a promising potential application for rapid and early clinical diagnosis of HIV infection.
早期诊断 HIV 生物标志物或基因是降低获得性免疫缺陷综合征（AIDS）死亡率的关键。在我们的工作中，我们开发了一种新的聚合酶链反应-动态光散射（PCR-DLS）分析方法，用于基于金纳米粒子（AuNPs）平均直径变化的一步灵敏检测 HIV DNA。这是第一个利用 DLS 技术作为信号读出的 PCR 分析方法，DLS 测量的粒径随目标 DNA 浓度的增加而增加。借助 AuNP 探针，该 PCR-DLS 分析方法可以有效地提高 PCR 反应的特异性，从而大大提高检测灵敏度，检测限为 1.8 aM（S/N = 3）。此外，该策略已成功用于分析人血清样品中的靶 DNA，表明 PCR-DLS 分析方法具有快速和早期临床诊断 HIV 感染的广阔应用前景。
