基于连接酶链反应的金纳米粒子组装用于超灵敏 DNA 检测。
Ligation Chain Reaction based gold nanoparticle assembly for ultrasensitive DNA detection.
机构信息
State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, JiangSu 214122, China; Institute of Plant Quarantine, Chinese Academy of Inspection and Quarantine, Beijing 100029, China.
出版信息
Biosens Bioelectron. 2014 Feb 15;52:8-12. doi: 10.1016/j.bios.2013.07.064. Epub 2013 Aug 14.
A simple and ultrasensitive DNA biosensor was developed utilizing the color and size changes originating from Ligation Chain Reaction (LCR)-based gold nanoparticle assembly as an indicator. Using synthetic DNA of 10-fold serial dilutions as a target, the limit of detection (LOD) was 1.5 aM with a range of DNA concentrations from 0.01 to 1000 fM by UV-vis absorption and 0.1 aM in the range of 0.01-10,000 fM by dynamic light scattering (DLS). This developed method is low-cost, rapid, and much lower LOD for the detection of specific short DNA sequences, and the results can be directly determined either by the color or by the DLS. The capability of high-throughput detection with the aid of PCR amplification apparatus can also be realized.
一种简单且超灵敏的 DNA 生物传感器是利用连接酶链反应（LCR）-基于金纳米粒子组装的颜色和大小变化开发的，作为指示剂。使用 10 倍系列稀释的合成 DNA 作为靶标，通过紫外可见吸收法，检测限（LOD）为 1.5 aM，DNA 浓度范围为 0.01 至 1000 fM，通过动态光散射（DLS）在 0.01 至 10,000 fM 的范围内为 0.1 aM。该方法成本低、速度快，对特定短 DNA 序列的检测具有更低的 LOD，并且可以通过颜色或 DLS 直接确定结果。借助 PCR 扩增仪器，还可以实现高通量检测。
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