Bostan B, Gevrek F, Balta O, Aytekin K, Asci M, Eren M B, Kuyucu Y E
Bratisl Lek Listy. 2018;119(10):630-635. doi: 10.4149/BLL_2018_112.
In this study, we sought to investigate the effect of different amounts of Bone Marrow-Derived Mesenchymal Stem Cells (BMSCs), obtained by different BMSCs, on the healing of avascular zone meniscal defects.
Treating avascular zone meniscal injuries has gained popularity. BMSCs contribute to the healing of avascular zone meniscal defects. The amount of BMSCs derived from different bone marrow stimulation techniques (BMSTs) varies, which could affect the therapeutic efficacy of this treatment.
Fifty-four skeletally mature female New Zealand White rabbits were used after local ethical committee approval. A full thickness, 1.5 mm diameter defect was produced in the inner two-thirds of the anterior portion of the medial meniscus avascular zone using a biopsy punch. Animals were enrolled into three different groups according to BMST (0.8 mm, 1.5 mm, and 4 mm). Medial menisci were harvested and prepared for histomorphometric, histologic and immune-histologic analyses.
Larger bridging tissues across the defect were detected in the 1.5-mm and 4-mm groups at 4 weeks (p < 0.05). The best quality score at the 1-,4- and 12-week endpoints was in 0.8 mm, 4 mm and 0.8 mm, 1.5 mm, respectively (p 0.05)CONCLUSION: The largest amount of BMSCs did not correlate with best quality and largest quantity of bridging tissue at the avascular zone in meniscal defects (Tab. 3, Fig. 4, Ref. 30).
在本研究中，我们试图探究通过不同方法获取的不同数量的骨髓间充质干细胞（BMSCs）对半月板无血管区缺损愈合的影响。
治疗半月板无血管区损伤越来越受到关注。骨髓间充质干细胞有助于半月板无血管区缺损的愈合。不同骨髓刺激技术（BMSTs）获得的骨髓间充质干细胞数量不同，这可能会影响该治疗的疗效。
经当地伦理委员会批准后，使用54只骨骼成熟的雌性新西兰白兔。使用活检打孔器在内侧半月板无血管区前部内三分之二处制造一个直径1.5毫米的全层缺损。根据骨髓刺激技术（0.8毫米、1.5毫米和4毫米）将动物分为三个不同的组。收集内侧半月板并准备进行组织形态计量学、组织学和免疫组织学分析。
在4周时，1.5毫米组和4毫米组中检测到跨越缺损的更大的桥接组织（p < 0.05）。在1周、4周和12周终点时，最佳质量评分分别在0.8毫米组、4毫米组以及0.8毫米组和1.5毫米组（p 0.05）。结论：在半月板缺损的无血管区，最大数量的骨髓间充质干细胞与最佳质量和最大数量的桥接组织并不相关（表3，图4，参考文献30）。
