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用二甲基亚砜预处理增强烟草细胞中低渗休克诱导的胞质和核酸钙浓度的升高。

Enhanced elevations of hypo-osmotic shock-induced cytosolic and nucleic calcium concentrations in tobacco cells by pretreatment with dimethyl sulfoxide.

作者信息

Nguyen Hieu T H, Bouteau François, Mazars Christian, Kuse Masaki, Kawano Tomonori

机构信息

a International Photosynthesis Industrialization Research Center, Faculty and Graduate School of Environmental Engineering , The University of Kitakyushu , Kitakyushu , Japan.

b Université Paris Diderot, Sorbonne Paris Cité , Laboratoire Interdisciplinaire des Energies de Demain , Paris , France.

出版信息

Biosci Biotechnol Biochem. 2019 Feb;83(2):318-321. doi: 10.1080/09168451.2018.1533801. Epub 2018 Oct 21.

Abstract

Dimethyl sulfoxide (DMSO) is a dipolar aprotic solvent widely used in biological assays. Here, we observed that DMSO enhanced the hypo-osmotically induced increases in the concentration of Ca in cytosolic and nucleic compartments in the transgenic cell-lines of tobacco (BY-2) expressing aequorin.

摘要

二甲基亚砜(DMSO)是一种广泛应用于生物测定的偶极非质子溶剂。在此,我们观察到二甲基亚砜增强了表达水母发光蛋白的烟草(BY-2)转基因细胞系中低渗诱导的胞质和细胞核区室中钙离子浓度的增加。

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