Department of Chemistry , University of Minnesota , 207 Pleasant Street SE , Minneapolis , Minnesota 55455 , United States.
Department of Chemistry and International Institute for Nanotechnology , Northwestern University , 2145 Sheridan Road , Evanston , Illinois 60208 , United States.
Anal Chem. 2018 Nov 20;90(22):13409-13418. doi: 10.1021/acs.analchem.8b03221. Epub 2018 Nov 1.
In this work, isothermal titration calorimetry (ITC) is employed as an affinity agent screening method for the surface-enhanced Raman scattering (SERS) detection of aflatoxin B1 (AFB1). AFB1, a potent carcinogen produced by a fungus that infects crops, is an important target due to the monitoring required based on its FDA regulation. Polymer affinity agents, like those studied here, have the potential to enable separation and detection of relevant small molecules such as pesticides, drugs, and biological toxins, like AFB1, especially when paired with a vibrational spectroscopy technique such as SERS. Herein, seven homopolymers were synthesized to be evaluated as AFB1 affinity agents based on hypothetical hydrogen bonding interactions. Nitrogen-inclusive poly( N-(2-aminoethyl) methacrylamide) (pAEMA) polymers and their oxygen analogs, poly(2-hydroxyethyl methacrylate) (pHEMA) were evaluated. ITC was demonstrated as an effective method for rapid screening among the polymer affinity agents. Chain lengths between seven and 39 repeat units were synthesized to study length-based variance in affinity agent performance. An ITC method was optimized and used for the rapid screening of polymer affinity agents. The results were compared to those generated by SERS. Good agreement between the ITC results and follow-up SERS sensing experiments showcased ITC's screening potential for analytical applications such as separation and detection.
在这项工作中，等温滴定量热法（ITC）被用作表面增强拉曼散射（SERS）检测黄曲霉毒素 B1（AFB1）的亲和剂筛选方法。AFB1 是一种由感染农作物的真菌产生的强致癌物质，由于根据美国 FDA 规定进行监测，因此成为一个重要目标。聚合物亲和剂，如这里研究的那些，有可能实现相关小分子（如农药、药物和生物毒素）的分离和检测，特别是与 SERS 等振动光谱技术结合使用时。在此，根据假设的氢键相互作用，合成了七种均聚物以评估其作为 AFB1 亲和剂的性能。评估了包含氮的聚（N-（2-氨基乙基）甲基丙烯酰胺）（pAEMA）聚合物及其氧类似物聚（2-羟乙基甲基丙烯酸酯）（pHEMA）。证明 ITC 是一种在聚合物亲和剂中进行快速筛选的有效方法。合成了具有 7 到 39 个重复单元的链长，以研究亲和剂性能的基于链长的变化。优化了 ITC 方法并用于聚合物亲和剂的快速筛选。将结果与 SERS 产生的结果进行了比较。ITC 结果与后续 SERS 传感实验之间的良好一致性展示了 ITC 在分离和检测等分析应用中的筛选潜力。
