Hirata Y, Hirose S, Takata S, Takagi Y, Matsubara H
Eur J Pharmacol. 1987 Mar 31;135(3):439-42. doi: 10.1016/0014-2999(87)90697-2.
Treatment of cultured rat vascular smooth muscle cells with human atrial natriuretic peptide (hANP) or Met(O)12hANP caused a similar and marked reduction (approximately 80%) of ANP receptor number (down-regulation). A second challenge with hANP stimulated the accumulation of intracellular cGMP in the down-regulated cells to the same extent as in control cells. These data suggest that ANP receptor sites are functionally heterogenous, the more abundant site being uncoupled from guanylate cyclase but susceptible to down-regulation.
用人心房利钠肽（hANP）或Met(O)12hANP处理培养的大鼠血管平滑肌细胞，会导致心房利钠肽受体数量出现相似且显著的减少（约80%）（下调）。用hANP再次刺激下调的细胞，细胞内cGMP的积累程度与对照细胞相同。这些数据表明，心房利钠肽受体位点在功能上是异质的，数量较多的位点与鸟苷酸环化酶解偶联，但易受下调影响。
