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抗衡离子塌缩及二胺对细菌视紫红质的影响。

Counterion collapse and the effect of diamines on bacteriorhodopsin.

作者信息

Marinetti T

出版信息

FEBS Lett. 1987 May 25;216(1):155-8. doi: 10.1016/0014-5793(87)80775-5.

DOI:10.1016/0014-5793(87)80775-5
PMID:3034670
Abstract

A recent report of electrical measurements on oriented bacteriorhodopsin in gels [(1986) FEBS Lett. 195, 164 168] concluded that low concentrations of diamines reversed the direction of the proton pump. Calculations are presented which show that in low diamine concentrations, charge displacements of the counterion atmosphere in the direction opposite to proton pumping are expected following H+ ejection. It is also shown that the effect will be sharply reduced by raising the diamine concentration or by adding excess salt, as was observed. Hence it is not necessary to conclude that diamines reverse the direction of the proton pump itself.

摘要

最近一篇关于凝胶中定向细菌视紫红质电测量的报告[(1986年)《欧洲生物化学学会联合会快报》195,164 - 168]得出结论,低浓度的二胺会使质子泵的方向反转。本文给出的计算表明,在低二胺浓度下,预计在H⁺排出后,抗衡离子气氛会沿与质子泵浦相反的方向发生电荷位移。还表明,正如所观察到的那样,提高二胺浓度或添加过量盐会使这种效应急剧降低。因此,没有必要得出二胺会使质子泵本身的方向反转的结论。

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FEBS Lett. 1987 May 25;216(1):155-8. doi: 10.1016/0014-5793(87)80775-5.
2
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引用本文的文献

1
Light-induced currents from oriented purple membrane: II. Proton and cation contributions to the photocurrent.紫膜光诱导电流:II.质子和阳离子对光电流的贡献。
Biophys J. 1990 May;57(5):951-63. doi: 10.1016/S0006-3495(90)82615-1.
2
Buffer effects on electric signals of light-excited bacteriorhodopsin.缓冲液对光激发细菌视紫红质电信号的影响。
Biophys J. 2000 Jun;78(6):3170-7. doi: 10.1016/S0006-3495(00)76853-6.
3
Effect of pH buffer molecules on the light-induced currents from oriented purple membrane.pH缓冲分子对来自定向紫膜的光诱导电流的影响。
Biophys J. 1991 Jul;60(1):204-16. doi: 10.1016/S0006-3495(91)82044-6.