Ohno K, Govindjee R, Ebrey T G
Biophys J. 1983 Aug;43(2):251-4. doi: 10.1016/S0006-3495(83)84347-1.
Proton pumping in closed vesicular systems containing bacteriorhodopsin that is initiated by an orange flash, is diminished by a subsequent blue flash. This blue light effect is due to light absorbed by the photocycle intermediate M412 (M), which was formed by the orange flash. A kinetic analysis of the blue-light-induced reduction of proton pumping shows that of the two components of M, only the slowly decaying component is involved in the reduction of proton movement. This may be the first correlation between a proton movement and a specific photochemical intermediate of bacteriorhodopsin. Furthermore, we report that blue light, acting on the slowly decaying intermediate, probably causes a movement of the protons in a direction opposite to that normally seen for light absorbed by bacteriorhodopsin.
在含有细菌视紫红质的封闭囊泡系统中，由橙色闪光引发的质子泵浦会被随后的蓝色闪光减弱。这种蓝光效应是由于光循环中间体M412（M）吸收了光，而M412是由橙色闪光形成的。对蓝光诱导的质子泵浦减少的动力学分析表明，在M的两个组分中，只有缓慢衰减的组分参与了质子运动的减少。这可能是质子运动与细菌视紫红质特定光化学中间体之间的首次关联。此外，我们报告称，作用于缓慢衰减中间体的蓝光可能会使质子的移动方向与细菌视紫红质吸收光时通常观察到的方向相反。
