蓝光对细菌视紫红质质子泵浦的影响。
Blue light effect on proton pumping by bacteriorhodopsin.
作者信息
Ohno K, Govindjee R, Ebrey T G
出版信息
Biophys J. 1983 Aug;43(2):251-4. doi: 10.1016/S0006-3495(83)84347-1.
Abstract
Proton pumping in closed vesicular systems containing bacteriorhodopsin that is initiated by an orange flash, is diminished by a subsequent blue flash. This blue light effect is due to light absorbed by the photocycle intermediate M412 (M), which was formed by the orange flash. A kinetic analysis of the blue-light-induced reduction of proton pumping shows that of the two components of M, only the slowly decaying component is involved in the reduction of proton movement. This may be the first correlation between a proton movement and a specific photochemical intermediate of bacteriorhodopsin. Furthermore, we report that blue light, acting on the slowly decaying intermediate, probably causes a movement of the protons in a direction opposite to that normally seen for light absorbed by bacteriorhodopsin.
摘要
在含有细菌视紫红质的封闭囊泡系统中，由橙色闪光引发的质子泵浦会被随后的蓝色闪光减弱。这种蓝光效应是由于光循环中间体M412（M）吸收了光，而M412是由橙色闪光形成的。对蓝光诱导的质子泵浦减少的动力学分析表明，在M的两个组分中，只有缓慢衰减的组分参与了质子运动的减少。这可能是质子运动与细菌视紫红质特定光化学中间体之间的首次关联。此外，我们报告称，作用于缓慢衰减中间体的蓝光可能会使质子的移动方向与细菌视紫红质吸收光时通常观察到的方向相反。
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本文引用的文献
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The quantum efficiency of proton pumping by the purple membrane of Halobacterium halobium.嗜盐菌紫膜质子泵的量子效率。
Biophys J. 1980 May;30(2):231-42. doi: 10.1016/S0006-3495(80)85091-0.
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Environmental effects on formation and photoreaction of the M412 photoproduct of bacteriorhodopsin: implications for the mechanism of proton pumping.环境对细菌视紫红质M412光产物形成及光反应的影响：对质子泵浦机制的启示
Biochemistry. 1981 Feb 3;20(3):649-55. doi: 10.1021/bi00506a031.
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Kinetics and stoichiometry of light-induced proton release and uptake from purple membrane fragments, Halobacterium halobium cell envelopes, and phospholipid vesicles containing oriented purple membrane.光诱导紫色膜片段、嗜盐菌细胞包膜以及含有定向紫色膜的磷脂囊泡中质子释放与摄取的动力学及化学计量学
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