寡核苷酸中可交换质子的吸收模式二维核Overhauser效应光谱学
Absorption mode two-dimensional NOE spectroscopy of exchangeable protons in oligonucleotides.
作者信息
Sklenár V, Brooks B R, Zon G, Bax A
出版信息
FEBS Lett. 1987 Jun 1;216(2):249-52. doi: 10.1016/0014-5793(87)80699-3.
PMID:3034676
Abstract
A new NMR method is described for the generation of absorption mode two-dimensional NOE spectra of oligonucleotides in H2O solution. The method yields spectra that are free of baseline distortions with excellent suppression of the intense H2O resonance. The method is demonstrated for a sample of the dodecamer d(CGCGAATTCGCG)2. All exchangeable base protons are identified and a number of new types of NOE connectivities are observed.
摘要
描述了一种新的核磁共振（NMR）方法，用于生成H₂O溶液中寡核苷酸的吸收模式二维核Overhauser效应（NOE）光谱。该方法产生的光谱没有基线失真，对强烈的H₂O共振有出色的抑制效果。以十二聚体d(CGCGAATTCGCG)₂样品为例展示了该方法。所有可交换的碱基质子都被识别出来，并观察到了一些新型的NOE连接性。
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