• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

溶液中d-(AATTGCAATT)自互补寡聚脱氧核苷酸的结构、碱基对打开动力学及质子交换机制研究

Study of structure, base-pair opening kinetics and proton exchange mechanism of the d-(AATTGCAATT) self-complementary oligodeoxynucleotide in solution.

作者信息

Kochoyan M, Lancelot G, Leroy J L

机构信息

Groupe de Biophysique, Ecole Polytechnique, Palaiseau, France.

出版信息

Nucleic Acids Res. 1988 Aug 11;16(15):7685-702. doi: 10.1093/nar/16.15.7685.

DOI:10.1093/nar/16.15.7685
PMID:2842732
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC338435/
Abstract

Using proton magnetic resonance, we have investigated the structure and the base-pair opening kinetics of the d-(AATTGCAATT) self-complementary duplex. All the non-exchangeable (except H5',5") and most exchangeable proton resonances have been assigned. The structure belongs to the B family. Imino proton exchange, measured by line broadening, longitudinal relaxation and magnetization transfer from water, is catalyzed by proton acceptors. The base-pair lifetimes, obtained by extrapolation of the exchange times to infinite concentration of ammonia are 2 and 3 milliseconds for internal A.Ts and 18 ms for G.C at 15 degrees C. In the absence of added catalysts, the imino proton of the first A.T base pair exchanges faster than that of the unpaired thymidine of the duplex formed by the sequence d-(AATTGCAATTT). This gives strong evidence for intrinsic exchange catalysis. The exchange of adenine amino protons from the closed state has been observed. Hence amino proton exchange is ill-suited for the investigation of base-pair opening kinetics.

摘要

利用质子磁共振技术，我们研究了d-(AATTGCAATT)自互补双链体的结构和碱基对打开动力学。所有不可交换（除H5',5"外）和大多数可交换质子共振峰均已归属。该结构属于B族。通过谱线展宽、纵向弛豫以及从水中的磁化转移测量的亚氨基质子交换由质子受体催化。在15℃下，通过将交换时间外推至氨的无限浓度得到的内部A.T碱基对的碱基对寿命为2和3毫秒，G.C碱基对的寿命为18毫秒。在没有添加催化剂的情况下，第一个A.T碱基对的亚氨基质子比由序列d-(AATTGCAATTT)形成的双链体中未配对胸腺嘧啶的亚氨基质子交换得更快。这为内在交换催化提供了有力证据。已观察到腺嘌呤氨基质子从封闭状态的交换。因此，氨基质子交换不适用于研究碱基对打开动力学。

相似文献

1
Study of structure, base-pair opening kinetics and proton exchange mechanism of the d-(AATTGCAATT) self-complementary oligodeoxynucleotide in solution.溶液中d-(AATTGCAATT)自互补寡聚脱氧核苷酸的结构、碱基对打开动力学及质子交换机制研究
Nucleic Acids Res. 1988 Aug 11;16(15):7685-702. doi: 10.1093/nar/16.15.7685.
2
Characterization of base-pair opening in deoxynucleotide duplexes using catalyzed exchange of the imino proton.利用亚氨基质子的催化交换对脱氧核苷酸双链体中的碱基对打开进行表征。
J Mol Biol. 1988 Mar 20;200(2):223-38. doi: 10.1016/0022-2836(88)90236-7.
3
Proton exchange and base-pair lifetimes in a deoxy-duplex containing a purine-pyrimidine step and in the duplex of inverse sequence.在含有嘌呤-嘧啶步的脱氧双链体以及反向序列双链体中的质子交换和碱基对寿命。
J Mol Biol. 1987 Aug 5;196(3):599-609. doi: 10.1016/0022-2836(87)90036-2.
4
Base-catalysis of imino proton exchange in DNA: effects of catalyst upon DNA structure and dynamics.DNA中亚氨基质子交换的碱催化作用：催化剂对DNA结构和动力学的影响。
Biochemistry. 1996 Jun 25;35(25):8439-49. doi: 10.1021/bi952932z.
5
Imino proton exchange in DNA catalyzed by ammonia and trimethylamine: evidence for a secondary long-lived open state of the base pair.氨和三甲胺催化的DNA中的亚氨基质子交换：碱基对二级长寿命开放状态的证据。
Biochemistry. 2000 Jan 25;39(3):607-15. doi: 10.1021/bi991863b.
6
Kinetics for exchange of imino protons in the d(C-G-C-G-A-A-T-T-C-G-C-G) double helix and in two similar helices that contain a G . T base pair, d(C-G-T-G-A-A-T-T-C-G-C-G), and an extra adenine, d(C-G-C-A-G-A-A-T-T-C-G-C-G).d（C-G-C-G-A-A-T-T-C-G-C-G）双螺旋以及包含一个G·T碱基对的两个类似螺旋d（C-G-T-G-A-A-T-T-C-G-C-G）和一个额外腺嘌呤的d（C-G-C-A-G-A-A-T-T-C-G-C-G）中亚氨基质子交换的动力学
Biochemistry. 1982 Dec 7;21(25):6567-74. doi: 10.1021/bi00268a038.
7
Alteration of A.T base-pair opening kinetics by the ammonium cation in DNA A-tracts.铵阳离子对DNA A序列中A·T碱基对打开动力学的改变。
Biochemistry. 2002 Oct 15;41(41):12467-74. doi: 10.1021/bi020184p.
8
Terminal base pairs of oligodeoxynucleotides: imino proton exchange and fraying.寡脱氧核苷酸的末端碱基对：亚氨基质子交换与解链
Biochemistry. 1995 Aug 22;34(33):10652-9. doi: 10.1021/bi00033a041.
9
A single mode of DNA base-pair opening drives imino proton exchange.单一模式的DNA碱基对打开驱动亚氨基质子交换。
Nature. 1987;328(6125):89-92. doi: 10.1038/328089a0.
10
Acid multimers of oligodeoxycytidine strands: stoichiometry, base-pair characterization, and proton exchange properties.寡聚脱氧胞苷链的酸性多聚体：化学计量、碱基对特征及质子交换特性
Biochemistry. 1993 Jun 15;32(23):6019-31. doi: 10.1021/bi00074a013.

引用本文的文献

1
How to benchmark RNA secondary structure prediction accuracy.如何评估 RNA 二级结构预测准确性。
Methods. 2019 Jun 1;162-163:60-67. doi: 10.1016/j.ymeth.2019.04.003. Epub 2019 Apr 2.
2
Design and Properties of Ligand-Conjugated Guanine Oligonucleotides for Recovery of Mutated G-Quadruplexes.配体偶联鸟嘌呤寡核苷酸的设计与性质及其用于恢复突变 G-四链体。
Molecules. 2018 Dec 6;23(12):3228. doi: 10.3390/molecules23123228.
3
Improving RNA nearest neighbor parameters for helices by going beyond the two-state model.通过超越二态模型来改进螺旋体的 RNA 最近邻参数。
Nucleic Acids Res. 2018 Jun 1;46(10):4883-4892. doi: 10.1093/nar/gky270.
4
Free-energy landscape and characteristic forces for the initiation of DNA unzipping.DNA解链起始的自由能景观与特征力
Biophys J. 2015 Apr 7;108(7):1727-1738. doi: 10.1016/j.bpj.2015.01.025.
5
Thermodynamics of RNA melting, one base pair at a time.逐个碱基解析 RNA 热变性的热力学
RNA. 2010 Sep;16(9):1687-91. doi: 10.1261/rna.2235010. Epub 2010 Jul 21.
6
In vitro synthesis of uniform poly(dG)-poly(dC) by Klenow exo- fragment of polymerase I.用聚合酶I的克列诺外切酶片段进行均匀聚（dG）-聚（dC）的体外合成。
Nucleic Acids Res. 2005 Jan 26;33(2):525-35. doi: 10.1093/nar/gki178. Print 2005.
7
Proton exchange and base-pair opening kinetics in 5'-d(CGCGAATTCGCG)-3' and related dodecamers.5'-d(CGCGAATTCGCG)-3'及相关十二聚体中的质子交换和碱基对打开动力学
Nucleic Acids Res. 1990 Feb 25;18(4):821-7. doi: 10.1093/nar/18.4.821.

本文引用的文献

1
Synthesis and structural studies of a self-complementary decadeoxynucleotide d(AATTGCAATT). II.-Proton magnetic resonance studies.自互补十聚脱氧核苷酸d(AATTGCAATT)的合成与结构研究。II. -质子磁共振研究。
Biochimie. 1981 Oct;63(10):785-90. doi: 10.1016/s0300-9084(81)80038-7.
2
Synthesis and structural studies of a self-complementary decadeoxynucleotide d(AATTGCAATT). I.-Synthesis and chemical characterization of the decanucleotide.一种自我互补的十脱氧核苷酸d(AATTGCAATT)的合成与结构研究。I. -十核苷酸的合成与化学表征。
Biochimie. 1981 Oct;63(10):775-84. doi: 10.1016/s0300-9084(81)80037-5.
3
Conformations of A-DNA and B-DNA in agreement with fiber X-ray and infrared dichroism.与纤维X射线和红外二色性一致的A-DNA和B-DNA构象。
Nucleic Acids Res. 1983 Mar 25;11(6):1897-908. doi: 10.1093/nar/11.6.1897.
4
Sequential resonance assignments in 1H NMR spectra of oligonucleotides by two-dimensional NMR spectroscopy.通过二维核磁共振光谱对寡核苷酸的1H NMR光谱进行顺序共振归属。
Biochemistry. 1984 Mar 27;23(7):1371-6. doi: 10.1021/bi00302a006.
5
Complete assignment of the non-exchangeable proton nmr resonances of [d-(GGAATTCC)]2 using two-dimensional nuclear overhauser effect spectra.利用二维核Overhauser效应谱对[d-(GGAATTCC)]₂的非交换质子核磁共振共振进行完全归属。
Biochem Biophys Res Commun. 1984 Mar 15;119(2):663-70. doi: 10.1016/s0006-291x(84)80301-0.
6
A one- and two-dimensional NMR study of the B to Z transition of (m5dC-dG)3 in methanolic solution.甲醇溶液中(m5dC-dG)3从B型到Z型转变的一维和二维核磁共振研究。
Nucleic Acids Res. 1984 Jan 25;12(2):1243-63. doi: 10.1093/nar/12.2.1243.
7
Two-dimensional proton nuclear magnetic resonance investigation of the synthetic deoxyribonucleic acid decamer d(ATATCGATAT)2.合成脱氧核糖核酸十聚体d(ATATCGATAT)₂的二维质子核磁共振研究
Biochemistry. 1983 Dec 6;22(25):5943-51. doi: 10.1021/bi00294a038.
8
A hydrogen exchange study of the open segment in a DNA double helix.DNA双螺旋中开放片段的氢交换研究。
Biochim Biophys Acta. 1984 Mar 22;798(1):46-52. doi: 10.1016/0304-4165(84)90008-4.
9
Sequential assignment of the 1H and 31P resonances of the double stranded deoxynucleotide d (ATGCAT)2 by 2D-NMR correlation spectroscopy.通过二维核磁共振相关光谱对双链脱氧核苷酸d(ATGCAT)₂的¹H和³¹P共振进行顺序归属。
Biochem Biophys Res Commun. 1984 Nov 14;124(3):774-83. doi: 10.1016/0006-291x(84)91025-8.
10
Nuclear magnetic resonance study of d-TGGCCA in solution.溶液中d-TGGCCA的核磁共振研究。
Nucleic Acids Res. 1984 Jul 25;12(14):5927-41. doi: 10.1093/nar/12.14.5927.