脐带血来源细胞产品中的细胞因子：对骨修复的机制性见解。
Cytokines in umbilical cord blood-derived cellular product: a mechanistic insight into bone repair.
作者信息
Sane Mukta S, Misra Neha, Mousa Omid Mohammad, Czop Steve, Tang Huiyuan, Khoo Larry T, Jones Christopher D, Mustafi Soumyajit Banerjee
机构信息
Department of Research & Development, Burst Biologics, Boise, ID 83705, USA.
Biomolecular Research Center, Boise State University, Boise, ID, 83725, USA.
出版信息
Regen Med. 2018 Dec;13(8):881-898. doi: 10.2217/rme-2018-0102. Epub 2018 Oct 22.
AIM
Umbilical cord blood (UCB) finds frequent applications in regenerative medicine. We evaluated the role of cytokines present in a uniquely processed, UCB-derived cellular allograft product (UCBp).
MATERIALS & METHODS: Luminex multiplex assay and standard cell biology methods were employed.
RESULTS
Study with allografts from 33 donors identified 44 quantifiable cytokines in the UCBp derived conditioned media (CM). The UCBp-CM elevated proliferation and migration rates of mesenchymal stem cells (MSCs) and bone marrow stromal cells. Moreover, UCBp-CM induced secretion of VEGF-A and osteoprotegerin, which promoted angiogenesis of endothelial cells and positively influenced the osteogenic differentiation of MSCs, respectively.
CONCLUSION
Cytokines in UCBp stimulate cellular processes important for bone regeneration, making UCBp an excellent candidate for potential applications in orthopedic procedures like bone non-union and spinal fusion.
目的
脐带血（UCB）在再生医学中应用广泛。我们评估了一种经过独特处理的、源自脐带血的细胞同种异体移植产品（UCBp）中细胞因子的作用。
材料与方法
采用Luminex多重检测法和标准细胞生物学方法。
结果
对来自33名供体的同种异体移植物进行的研究在UCBp来源的条件培养基（CM）中鉴定出44种可定量的细胞因子。UCBp-CM提高了间充质干细胞（MSC）和骨髓基质细胞的增殖和迁移率。此外，UCBp-CM诱导了VEGF-A和骨保护素的分泌，分别促进了内皮细胞的血管生成并对MSC的成骨分化产生了积极影响。
结论
UCBp中的细胞因子刺激了对骨再生重要的细胞过程，使UCBp成为骨不连和脊柱融合等骨科手术潜在应用的优秀候选者。
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