Orthopaedic Biomechanics, Department of Biomedical Engineering and Institute for Complex Molecular Systems, Eindhoven University of Technology, P.O. Box 513, 5600 MB, Eindhoven, the Netherlands.
Curr Osteoporos Rep. 2021 Feb;19(1):88-100. doi: 10.1007/s11914-020-00648-6. Epub 2021 Jan 15.
One aim in bone tissue engineering is to develop human cell-based, 3D in vitro bone models to study bone physiology and pathology. Due to the heterogeneity of cells among patients, patient's own cells are needed to be obtained, ideally, from one single cell source. This review attempts to identify the appropriate cell sources for development of such models.
Bone marrow and peripheral blood are considered as suitable sources for extraction of osteoblast/osteocyte and osteoclast progenitor cells. Recent studies on these cell sources have shown no significant differences between isolated progenitor cells. However, various parameters such as medium composition affect the cell's proliferation and differentiation potential which could make the peripheral blood-derived stem cells superior to the ones from bone marrow. Peripheral blood can be considered a suitable source for osteoblast/osteocyte and osteoclast progenitor cells, being less invasive for the patient. However, more investigations are needed focusing on extraction and differentiation of both cell types from the same donor sample of peripheral blood.
骨组织工程的一个目标是开发基于人细胞的 3D 体外骨模型，以研究骨生理学和病理学。由于患者之间细胞的异质性，需要获得患者自身的细胞，理想情况下，应从单一细胞来源获得。本综述试图确定用于开发此类模型的合适细胞来源。
骨髓和外周血被认为是提取成骨细胞/成骨细胞和破骨细胞祖细胞的合适来源。这些细胞来源的最新研究表明，分离的祖细胞之间没有显著差异。然而，各种参数（如培养基组成）会影响细胞的增殖和分化潜能，这可能使外周血来源的干细胞优于骨髓来源的干细胞。外周血可以被认为是成骨细胞/成骨细胞和破骨细胞祖细胞的合适来源，对患者的创伤较小。然而，仍需要进行更多的研究，重点是从外周血的同一供体样本中提取和分化这两种细胞类型。
