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[单纯疱疹病毒胸苷激酶基因对多种胸苷激酶缺陷型人和动物细胞系转化的比较研究]

[Comparative study of the transformation of various thymidine kinase-deficient human and animal cell lines with the thymidine kinase gene of the Herpes simplex virus].

作者信息

Grinenko N F, Al'tshteĭn A L

出版信息

Genetika. 1987 Apr;23(4):670-6.

PMID:3034725
Abstract

The comparative study of transformation of four thymidine kinase deficient cell lines (mouse mammary carcinoma cell line FS tk-; rat cell line Rat-2tk-; mouse cell line Ltk-, clone D1; human cell line 143tk-) with the thymidine kinase cloned gene of Herpes simplex virus 1 was undertaken. The differences in efficiency and optimal conditions of transformation were shown for these cell lines. The advantages and disadvantages of the cell systems examined for the use in experiments for transformation and cotransformation of cultured cells with isolated genes are discussed.

摘要

对四种胸苷激酶缺陷细胞系（小鼠乳腺癌细胞系FS tk-；大鼠细胞系Rat-2tk-；小鼠细胞系Ltk-，克隆D1；人细胞系143tk-）用单纯疱疹病毒1的胸苷激酶克隆基因进行转化的比较研究。展示了这些细胞系在转化效率和最佳条件方面的差异。讨论了所检测的细胞系统用于培养细胞与分离基因的转化和共转化实验的优缺点。

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Genetika. 1987 Apr;23(4):670-6.
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