单纯疱疹病毒1型胸苷激酶基因在大肠杆菌K12中的克隆：一种用于基因导入动物细胞的选择标记。
Cloning of the herpes simplex virus type 1 thymidine kinase gene in E. coli K 12: a selective marker for gene transfer into animal cells.
作者信息
Colbere-Garapin F, Horodniceanu F, Kourilsky P, Garapin A C
出版信息
Dev Biol Stand. 1980;46:75-82.
PMID:6245004
Abstract
The Herpes Simplex Virus type 1 (HSV 1) thymidine kinase (TK) gene, carried by a 3.6 kb Bam H1 DNA fragment, was isolated and ligated to Bam H1 cleaved bacterial plasmid pBR 322. The TK gene, cloned in E. coli, is functional when it is transferred back into Eukaryotic cells, and can thus be used as a selective marker for gene transfer.
摘要
由一个3.6 kb的Bam H1 DNA片段携带的单纯疱疹病毒1型（HSV 1）胸苷激酶（TK）基因被分离出来，并与经Bam H1切割的细菌质粒pBR 322连接。克隆于大肠杆菌中的TK基因，当被转回真核细胞时具有功能，因此可作为基因转移的选择标记。
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