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Mason-Pfizer 猴病毒包膜糖蛋白循环及其与不成熟颗粒的囊泡共转运。

Mason-Pfizer Monkey Virus Envelope Glycoprotein Cycling and Its Vesicular Co-Transport with Immature Particles.

机构信息

Department of Biochemistry and Microbiology, University of Chemistry and Technology, 166 28 Prague, Czech Republic.

Imaging methods core facility at BIOCEV, Faculty of Science, Charles University, 252 50 Prague, Czech Republic.

出版信息

Viruses. 2018 Oct 20;10(10):575. doi: 10.3390/v10100575.

DOI:10.3390/v10100575
PMID:30347798
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6212865/
Abstract

The envelope glycoprotein (Env) plays a crucial role in the retroviral life cycle by mediating primary interactions with the host cell. As described previously and expanded on in this paper, Env mediates the trafficking of immature Mason-Pfizer monkey virus (M-PMV) particles to the plasma membrane (PM). Using a panel of labeled RabGTPases as endosomal markers, we identified Env mostly in Rab7a- and Rab9a-positive endosomes. Based on an analysis of the transport of recombinant fluorescently labeled M-PMV Gag and Env proteins, we propose a putative mechanism of the intracellular trafficking of M-PMV Env and immature particles. According to this model, a portion of Env is targeted from the trans-Golgi network (TGN) to Rab7a-positive endosomes. It is then transported to Rab9a-positive endosomes and back to the TGN. It is at the Rab9a vesicles where the immature particles may anchor to the membranes of the Env-containing vesicles, preventing Env recycling to the TGN. These Gag-associated vesicles are then transported to the plasma membrane.

摘要

包膜糖蛋白（Env）在逆转录病毒生命周期中起着至关重要的作用，通过介导与宿主细胞的主要相互作用来实现。如前所述，并在本文中进一步扩展，Env 介导不成熟的 Mason-Pfizer 猴病毒（M-PMV）颗粒向质膜（PM）的运输。使用一系列标记的 RabGTPases 作为内体标记物，我们发现 Env 主要存在于 Rab7a 和 Rab9a 阳性内体中。基于对重组荧光标记的 M-PMV Gag 和 Env 蛋白运输的分析，我们提出了 M-PMV Env 和不成熟颗粒的细胞内运输的假设机制。根据该模型，Env 的一部分从高尔基体到 Rab7a 阳性内体被靶向。然后，它被运送到 Rab9a 阳性内体并返回高尔基体。正是在 Rab9a 小泡中，不成熟的颗粒可能锚定到含有 Env 的小泡的膜上，防止 Env 再循环到高尔基体。这些与 Gag 相关的小泡随后被运送到质膜。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/253b/6212865/086f90b11d5f/viruses-10-00575-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/253b/6212865/66e690ac1a42/viruses-10-00575-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/253b/6212865/d3281148884e/viruses-10-00575-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/253b/6212865/56224e368434/viruses-10-00575-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/253b/6212865/f3b729374ffe/viruses-10-00575-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/253b/6212865/4ca031bc90b8/viruses-10-00575-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/253b/6212865/6096748b776b/viruses-10-00575-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/253b/6212865/33b2d87fcfa0/viruses-10-00575-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/253b/6212865/fbc40bb2f87f/viruses-10-00575-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/253b/6212865/e0283b833504/viruses-10-00575-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/253b/6212865/ff5902e9c644/viruses-10-00575-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/253b/6212865/086f90b11d5f/viruses-10-00575-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/253b/6212865/66e690ac1a42/viruses-10-00575-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/253b/6212865/d3281148884e/viruses-10-00575-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/253b/6212865/56224e368434/viruses-10-00575-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/253b/6212865/f3b729374ffe/viruses-10-00575-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/253b/6212865/4ca031bc90b8/viruses-10-00575-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/253b/6212865/6096748b776b/viruses-10-00575-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/253b/6212865/33b2d87fcfa0/viruses-10-00575-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/253b/6212865/fbc40bb2f87f/viruses-10-00575-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/253b/6212865/e0283b833504/viruses-10-00575-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/253b/6212865/ff5902e9c644/viruses-10-00575-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/253b/6212865/086f90b11d5f/viruses-10-00575-g011.jpg

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