Xiong Hai-Rong, Li Cong, He Chun-Xi, He Yu-Min, Yuan Cheng-Fu, Wang Ting, Zhang Chang-Cheng, Yuan Ding, Liu Chao-Qi
Hubei Key Laboratory of Tumor Microenvironment and Immunotheraphy, Three Gorges University， Yichang 443002, China.
Medical College， Three Gorges University， Yichang 443002， China.
Zhongguo Zhong Yao Za Zhi. 2018 Sep;43(17):3525-3529. doi: 10.19540/j.cnki.cjcmm.20180606.001.
To research the effection and probable mechanism for the total saponins of Panax japonicas(TPSJ) in mice on non-alcoholic fatty liver disease. Forty SPF male Kunming mice were randomily divided into four group:control group,NAFLD group, low-dose TPSJ treated group,high-dose TPSJ treated group. High-fatty and high-frutose-diet was applied to eatablish NAFLD model，and TPSJ (100 and 200 mg·kg⁻¹) in feeding were given for the TPSJ groups for 4 weeks. To collect the serum with liver and the ALT and TC of serum were monitored after 4 weeks. The hepatic histopathologic structure was observed by haematoxylin-eosin (HE) staining, RT-PCR and RT-qPCR was applied for the detection of miR-199-5p，VEGFa，HGF，c-Met and protein expression level was detected bv laser confocal microscope.Compared with control group, the level of serum ALT and TC in the model group was higher,the liver of the model group showed that hepatocytes display obvious lipid deposition. Then TPSJ treated showed that markedly improved histopathologic changes, decreased fatty deposition. In the meantime,the expression level of miR-199-5p was significantly decreased, thus the expression of HGF and c-Met were significantly increased. TPSJ play a role of prevention on fatty liver, the machanism maybe by blocking miR-199-5p targeted to c-Met signaling pathways in NAFLD.
研究竹节参总皂苷（TPSJ）对小鼠非酒精性脂肪性肝病的影响及可能机制。将40只SPF级雄性昆明小鼠随机分为四组：对照组、非酒精性脂肪性肝病组、低剂量TPSJ治疗组、高剂量TPSJ治疗组。采用高脂高糖饮食建立非酒精性脂肪性肝病模型，TPSJ组给予100和200mg·kg⁻¹的TPSJ灌胃4周。4周后采集肝脏及血清，检测血清谷丙转氨酶（ALT）和总胆固醇（TC）。苏木精-伊红（HE）染色观察肝脏组织病理结构，采用逆转录-聚合酶链反应（RT-PCR）和实时荧光定量PCR（RT-qPCR）检测miR-199-5p、血管内皮生长因子a（VEGFa）、肝细胞生长因子（HGF）、c-Met，激光共聚焦显微镜检测蛋白表达水平。与对照组相比，模型组血清ALT和TC水平升高，模型组肝脏显示肝细胞有明显脂质沉积。TPSJ治疗后组织病理学改变明显改善，脂肪沉积减少。同时，miR-199-5p表达水平显著降低，HGF和c-Met表达显著增加。TPSJ对脂肪肝有预防作用，其机制可能是通过阻断非酒精性脂肪性肝病中miR-199-5p靶向c-Met信号通路。
