竹节人参总皂苷对棕榈酸诱导的HepG2细胞凋亡的保护作用
[Protective effects of total saponins of Panax japonicas on HepG2 cell apoptosis induced with palmitic acid].
作者信息
Tu Hao, Zhou Qin, Zhang Xun, Wang Ting, Yuan Cheng-Fu, Zhang Chang-Cheng, Yuan Ding, Liu Zhao-Qi
机构信息
Medical College, Three Gorges University, Yichang 443002, China.
Renhe Hospital, Three Gorges University, Yichang 443001, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2018 Jan;43(2):390-395. doi: 10.19540/j.cnki.cjcmm.20171027.011.
This study aimed to investigate the molecular mechanism and protective effect of total saponins of Panax japonicas (TSPJ) on HepG2 cells apoptosis induced by palmitic acid (PA).The HepG2 cells were cultured , and divided into five groups: the control group, the model group, the high-dose group (50 mg·L⁻¹), the middle-dose group (25 mg·L⁻¹) and the low-dose group (12.5 mg·L⁻¹).The cells of the five groups were cultured continuously for 24 hours. The cell viability was measured with MTT. HepG2 cells apoptosis was detected by Hoechest staining and Annexin V-FITC/PI staining. The protein expressions of BCL-2, CHOP and TLR4 were measured with western blotting and flow cytometry analysis. The mRNA expressions of TNF-α, IL-1β, BCL-2, CHOP and GAPDH were measured with RT-PCR. The results suggested that compared with the control group, the number of HepG2 cells of the model group were reduced significantly (<0.01), while the number of apoptotic HepG2 cells were increased. Compared with the model group, the number of HepG2 cells of the high-dose group and the middle-dose group were increased significantly (<0.01), whereas the number of apoptotic HepG2 cells were reduced. Compared with the control group, TNF-α, IL-1β and CHOP mRNA expressions and CHOP and TLR4 protein expressions in the model group were significantly up-regulated (<0.01), while BCL-2 protein and mRNA expressions in the model group were significantly decreased (<0.01). Compared with the model group, TNF-α, IL-1β and CHOP mRNA expressions and CHOP and TLR4 protein expressions in the high-dose group were significantly decreased (<0.01), while BCL-2 protein and mRNA expressions in the high-dose group were significantly up-regulated (<0.01).In conclusion, TSPJ can reduce inflammation and apoptosis induced by palmitic acid, with a certain protective effect on liver cells.
本研究旨在探讨竹节参总皂苷（TSPJ）对棕榈酸（PA）诱导的HepG2细胞凋亡的分子机制及保护作用。培养HepG2细胞，并将其分为五组：对照组、模型组、高剂量组（50 mg·L⁻¹）、中剂量组（25 mg·L⁻¹）和低剂量组（12.5 mg·L⁻¹）。五组细胞连续培养24小时。用MTT法检测细胞活力。通过Hoechest染色和Annexin V-FITC/PI染色检测HepG2细胞凋亡情况。用蛋白质印迹法和流式细胞术分析检测BCL-2、CHOP和TLR4的蛋白表达。用RT-PCR检测TNF-α、IL-1β、BCL-2、CHOP和GAPDH的mRNA表达。结果表明，与对照组相比，模型组HepG2细胞数量显著减少（<0.01），而凋亡的HepG2细胞数量增加。与模型组相比，高剂量组和中剂量组HepG2细胞数量显著增加（<0.01），而凋亡的HepG2细胞数量减少。与对照组相比，模型组TNF-α、IL-1β和CHOP的mRNA表达以及CHOP和TLR4的蛋白表达显著上调（<0.01），而模型组BCL-2的蛋白和mRNA表达显著下降（<0.01）。与模型组相比，高剂量组TNF-α、IL-1β和CHOP的mRNA表达以及CHOP和TLR4的蛋白表达显著下降（<0.01），而高剂量组BCL-2的蛋白和mRNA表达显著上调（<0.01）。综上所述，TSPJ可减轻棕榈酸诱导的炎症和凋亡，对肝细胞具有一定的保护作用。
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