用[F]ML-8对大鼠肺纤维化进行凋亡PET成像
Apoptotic PET Imaging of Rat Pulmonary Fibrosis With [F]ML-8.
作者信息
Xiong Ying, Nie Dahong, Liu Shaoyu, Ma Hui, Su Shu, Sun Aixia, Zhao Jing, Zhang Zhanwen, Xiang Xianhong, Tang Ganghua
机构信息
1 Guangdong Engineering Research Center for Translational Application of Medical Radiopharmaceuticals and Department of Medical Imaging, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
出版信息
Mol Imaging. 2018 Jan-Dec;17:1536012118795728. doi: 10.1177/1536012118795728.
OBJECTIVE
To investigate the value of 2-(3-[F]fluoropropyl)-2-methyl-malonic acid ([F]ML-8) positron emission tomography (PET) imaging of rat pulmonary fibrosis.
METHODS
Male Sprague-Dawley rats were divided into 2 groups, including pulmonary fibrosis model group and control group. The rat model was established by an intratracheal instillation of bleomycin (BLM). Control rats were treated with saline. Positron emission tomography/computed tomography (CT) with [F]ML-8 or F-fluorodeoxyglucose ([F]FDG) was performed on 2 groups. After PET/CT imaging, lung tissues were collected for histologic examination. Data were analyzed and comparisons between 2 groups were performed using Student t test.
RESULTS
Bleomycin-treated rats showed a higher lung uptake of [F]ML-8 than control rats ( P < .05). In BLM-treated rats, the lung to muscle relative uptake ratio of [F]ML-8 was also higher than that of [F]FDG ( P < .05). Pathological examination showed overproliferation of fibroblasts and deposition of collagen in lungs from BLM-treated rats. Compared to control rats, BLM-treated rats had higher lung hydroxyproline content ( P < .05). Immunofluorescence staining indicated more apoptotic cells in BLM-treated rats than those in control rats. Moreover, the apoptosis rate of lung tissues obtained from BLM-treated rats was higher than that from control rats ( P < .05).
CONCLUSIONS
2-(3-[F]fluoropropyl)-2-methyl-malonic acid PET/CT could be used for noninvasive diagnosis of pulmonary fibrosis in a rat model.
目的
探讨2-(3-[F]氟丙基)-2-甲基丙二酸（[F]ML-8）正电子发射断层扫描（PET）对大鼠肺纤维化的成像价值。
方法
将雄性Sprague-Dawley大鼠分为2组，即肺纤维化模型组和对照组。通过气管内注入博来霉素（BLM）建立大鼠模型。对照大鼠用生理盐水治疗。对2组大鼠进行[F]ML-8或F-氟脱氧葡萄糖（[F]FDG）正电子发射断层扫描/计算机断层扫描（CT）。PET/CT成像后，收集肺组织进行组织学检查。分析数据并使用Student t检验对2组进行比较。
结果
博来霉素处理的大鼠肺对[F]ML-8的摄取高于对照大鼠（P <.05）。在博来霉素处理的大鼠中，[F]ML-8的肺与肌肉相对摄取率也高于[F]FDG（P <.05）。病理检查显示博来霉素处理的大鼠肺中成纤维细胞过度增殖和胶原沉积。与对照大鼠相比，博来霉素处理的大鼠肺羟脯氨酸含量更高（P <.05）。免疫荧光染色表明博来霉素处理的大鼠凋亡细胞比对照大鼠多。此外，博来霉素处理的大鼠获得的肺组织凋亡率高于对照大鼠（P <.05）。
结论
2-(3-[F]氟丙基)-2-甲基丙二酸PET/CT可用于大鼠模型肺纤维化的无创诊断。
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