State Key Laboratory of Crop Biology, MOA Key Laboratory of Horticultural Crop Biology and Germplasm Innovation, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An, 271018, Shandong, China.
College of Biological and Agricultural Engineering, Weifang University, Weifang, 261061, Shandong, China.
Plant Sci. 2018 Nov;276:181-188. doi: 10.1016/j.plantsci.2018.08.017. Epub 2018 Aug 30.
Phytohormone ethylene is involved in salt stress response. As a key regulator of ethylene signaling, ethylene response factors (ERFs) have been reported to regulate salt stress tolerance. However, there are few studies on the relationship between ERFs in salt stress response. In this study, we isolated a salt-responsive gene MdERF4. Overexpression of MdERF4 negatively regulated salt stress tolerance and ethylene response, which was contrary to that of MdERF3 transgenic lines. Biochemical assays showed that MdERF4 directly bound to the DRE motif of MdERF3 promoter and suppressed its transcription. In addition, genetic analysis revealed that MdERF4 was involved in ethylene-mediated salt tolerance. Taken together, these findings demonstrated the transcriptional regulation between MdERF4 and MdERF3 in salt stress response and provided new insight into the ethylene-modulated salt stress response.
植物激素乙烯参与盐胁迫响应。作为乙烯信号转导的关键调节因子，乙烯应答因子（ERFs）已被报道调节盐胁迫耐受性。然而，关于 ERFs 在盐胁迫响应中的关系的研究较少。在这项研究中，我们分离出一个对盐响应的基因 MdERF4。MdERF4 的过表达负调控盐胁迫耐受性和乙烯反应，这与 MdERF3 转基因系的情况相反。生化分析表明，MdERF4 直接结合 MdERF3 启动子的 DRE 基序并抑制其转录。此外，遗传分析表明 MdERF4 参与乙烯介导的耐盐性。综上所述，这些结果表明了 MdERF4 和 MdERF3 在盐胁迫响应中的转录调控，并为乙烯调节盐胁迫响应提供了新的见解。
