State Key Laboratory of Crop Biology, MOA Key Laboratory of Horticultural Crop Biology and Germplasm Innovation, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An, Shandong, 271018, China.
College of Biological and Agricultural Engineering, Weifang University, Weifang, Shandong, 261061, China.
Physiol Plant. 2018 Nov;164(3):279-289. doi: 10.1111/ppl.12724. Epub 2018 Jul 2.
It is known that ethylene signaling is involved in the regulation of the salt stress response. However, the molecular mechanism of ethylene-regulated salt stress tolerance remains largely unclear. In this study, an apple NAM ATAF CUC transcription factor, MdNAC047, was isolated and functionally characterized to be involved in ethylene-modulated salt tolerance. MdNAC047 gene was significantly induced by salt treatment and its overexpression conferred increased tolerance to salt stress and facilitated the release of ethylene. Quantitative real-time-PCR analysis demonstrated that overexpression of MdNAC047 increased the expression of ethylene-responsive genes. Electrophoretic mobility shift assay, yeast one-hybrid and dual-luciferase assays suggested that MdNAC047 directly binds to the MdERF3 (ETHYLENE RESPONSE FACTOR) promoter and activates its transcription. In addition, genetic analysis assays indicated that MdNAC047 regulates ethylene production at least partially in an MdERF3-dependent pathway. Overall, we found a novel 'MdNAC047-MdERF3-ethylene-salt tolerance' regulatory pathway, which provide new insight into the link between ethylene and salt stress.
已知乙烯信号参与了盐胁迫反应的调节。然而，乙烯调控盐胁迫耐受的分子机制在很大程度上仍不清楚。本研究中，从苹果中分离并鉴定了一个 NAM、ATAF、CUC 转录因子 MdNAC047，其参与了乙烯调控的盐耐受性。MdNAC047 基因受盐处理显著诱导，过表达该基因可提高盐胁迫耐受性并促进乙烯的释放。定量实时 PCR 分析表明，MdNAC047 的过表达增加了乙烯响应基因的表达。电泳迁移率变动分析、酵母单杂交和双荧光素酶报告基因分析表明，MdNAC047 可直接结合到 MdERF3（ETHYLENE RESPONSE FACTOR）启动子并激活其转录。此外，遗传分析表明，MdNAC047 至少部分通过 MdERF3 依赖途径调控乙烯的产生。总的来说，我们发现了一个新的“MdNAC047-MdERF3-乙烯-盐耐受”调控通路，为乙烯和盐胁迫之间的联系提供了新的见解。
