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MdZAT5 的过表达调节苹果愈伤组织中花色苷的积累和耐盐性反应,MdZAT5 是一个 C2H2 型锌指蛋白。

Overexpression of MdZAT5, an C2H2-Type Zinc Finger Protein, Regulates Anthocyanin Accumulation and Salt Stress Response in Apple Calli and .

机构信息

National Key Laboratory of Crop Biology, National Research Center for Apple Engineering and Technology, College of Horticulture Science and Engineering, Shandong Agricultural University, Taian 271018, China.

College of Plant Protection, Shandong Agricultural University, Taian 271018, China.

出版信息

Int J Mol Sci. 2022 Feb 8;23(3):1897. doi: 10.3390/ijms23031897.

Abstract

Zinc finger proteins are widely involved and play an important role in plant growth and abiotic stress. In this research, , a gene encoding C2H2-type zinc finger protein, was cloned and investigated. The was highly expressed in flower tissues by qRT-PCR analyses and GUS staining. Promoter analysis showed that contained multiple response elements, and the expression levels of were induced by various abiotic stress treatments. Overexpression of in apple calli positively regulated anthocyanin accumulation by activating the expressions of anthocyanin biosynthesis-related genes. Overexpression of in also enhanced the accumulation of anthocyanin. In addition, increased the sensitivity to salt stress in apple calli. Ectopic expression of in reduced the expression of salt-stress-related genes ( and ) and improved the sensitivity to salt stress. In conclusion, these results suggest that plays a positive regulatory role in anthocyanin accumulation and negatively regulates salt resistance.

摘要

锌指蛋白广泛参与并在植物生长和非生物胁迫中发挥重要作用。本研究克隆并研究了一个编码 C2H2 型锌指蛋白的基因。qRT-PCR 分析和 GUS 染色表明,在花组织中高度表达。启动子分析表明,包含多个响应元件,并且表达水平通过各种非生物胁迫处理诱导。在苹果愈伤组织中过表达通过激活类黄酮生物合成相关基因的表达来正向调节花青苷的积累。在 中过表达也增强了花青苷的积累。此外,在苹果愈伤组织中增加了对盐胁迫的敏感性。在 中异位表达降低了盐胁迫相关基因(和)的表达并提高了对盐胁迫的敏感性。总之,这些结果表明在花青苷积累中起正调控作用,而在耐盐性中起负调控作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee18/8836528/15a3509c1e1c/ijms-23-01897-g001.jpg

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