Division of Gastroenterology, Department of Internal Medicine, Kobe University Graduate School of Medicine, Japan.
Department of Pharmaceutical Microbiology, Faculty of Life Sciences, Kumamoto University, Japan.
Talanta. 2019 Jan 15;192:182-188. doi: 10.1016/j.talanta.2018.09.047. Epub 2018 Sep 15.
Blood tests, which are used to evaluate health status, are relatively non-invasive and provide a great deal of health-related information. Blood is usually collected using a standard venous blood sampling protocol, but it is possible to collect blood from a subject's fingertip, and previous studies have investigated whether fingertip-derived blood can be used for various blood tests. In this study, the proteomes and metabolomes of venous and fingertip plasma were analyzed using non-targeted proteomics and metabolomics, respectively. In proteomics, the levels of 523 proteins were compared between venous and fingertip plasma. The correlation coefficient (r) for the relationship between protein levels of venous and fingertip plasma was 0.9999. Some proteins had high fingertip to venous plasma level ratios (finger:venous ratios), whereas others had low finger:venous ratios, and the mean±standard deviation (SD) finger:venous ratio was 0.994 ± 0.304. In metabolomics, 40, 33, and 216 cationic metabolites, anionic metabolites, and lipids, respectively, were detected in venous plasma, and the equivalent figures for fingertip plasma were 40, 35, and 216, respectively. Regarding the correlations between metabolite levels in venous and fingertip plasma, the correlation coefficients (r) for cationic metabolites, anionic metabolites, and lipids were 0.9952, 0.9699, and 0.9980, respectively. The mean±SD finger:venous ratio was 1.19 ± 0.584 for cationic metabolites, 1.23 ± 0.548 for anionic metabolites, and 1.00 ± 0.245 for lipids. Our study suggests that it might be possible to use fingertip plasma to measure plasma protein and metabolite levels, and will contribute to development of a fingertip blood sampling procedure for measuring blood biomarker levels.
血液检测用于评估健康状况，相对无创，并提供大量与健康相关的信息。通常使用标准的静脉采血方案采集血液，但也可以从受试者的指尖采集血液，之前的研究已经探讨了指尖血是否可用于各种血液检测。在这项研究中，分别使用非靶向蛋白质组学和代谢组学分析了静脉和指尖血浆的蛋白质组和代谢组。在蛋白质组学中，比较了静脉和指尖血浆中 523 种蛋白质的水平。静脉和指尖血浆中蛋白质水平的相关系数(r)为 0.9999。一些蛋白质的指尖与静脉血浆比值（指尖：静脉比值）较高，而另一些蛋白质的指尖：静脉比值较低，平均±标准差（SD）指尖：静脉比值为 0.994±0.304。在代谢组学中，分别在静脉血浆中检测到 40、33 和 216 种阳离子代谢物、阴离子代谢物和脂质，而在指尖血浆中检测到的相应数值分别为 40、35 和 216。关于静脉和指尖血浆中代谢物水平的相关性，阳离子代谢物、阴离子代谢物和脂质的相关系数(r)分别为 0.9952、0.9699 和 0.9980。阳离子代谢物、阴离子代谢物和脂质的平均±SD 指尖：静脉比值分别为 1.19±0.584、1.23±0.548 和 1.00±0.245。我们的研究表明，使用指尖血浆测量血浆蛋白质和代谢物水平可能是可行的，并将有助于开发用于测量血液生物标志物水平的指尖采血程序。
