Department of Chemistry, University of Turin, Via Giuria, 5, I-10125 Turin, Italy.
Department of Chemistry, University of Turin, Via Giuria, 5, I-10125 Turin, Italy.
Talanta. 2019 Jan 15;192:288-294. doi: 10.1016/j.talanta.2018.09.037. Epub 2018 Sep 18.
A multiplex Lateral Flow Immunoassay was developed based on the use of a single Test line and multicolour gold nanoparticles (GNPs) as signal reporters. Red and blue GNPs were linked to antibodies directed towards two different analytes and included in a typical lateral flow immunoassay configuration, in which the Test line was formed by the mixture of two antigens. As a result of the immunoreactions occurring at the Test zone, diverse combinations of red and blue GNPs labels were captured. Therefore, the Test line assumed different colours depending on which - and how much - analyte is present in the sample. The multiplexing capability of the 'colour-encoded assay' is illustrated by the simultaneous detection of aflatoxin B1 (AFB1) and type-B fumonisins (FMs) in wheat and food products that made with wheat. Reproducible detection of AFB1 and FMs contamination in raw and processed food was achieved with visual cut-off levels at 1 ng mL and 50 ng mL, respectively. The contaminant was identified based on the colour of the label according with a specific colour code. Furthermore, strips images were acquired by means of a common smartphone and analysed through RGB data analysis providing semi-quantitative detection of the two mycotoxins.
开发了一种基于单条测试线和多色金纳米粒子（GNPs）作为信号报告物的多重侧向流动免疫分析。红色和蓝色 GNPs 与针对两种不同分析物的抗体相连，并包含在典型的侧向流动免疫分析配置中，其中测试线由两种抗原的混合物形成。由于在测试区发生免疫反应，不同组合的红色和蓝色 GNPs 标签被捕获。因此，测试线的颜色取决于样品中存在的分析物的种类和数量。“颜色编码测定法”的多重检测能力通过同时检测小麦和用小麦制成的食品中的黄曲霉毒素 B1（AFB1）和 B 型伏马菌素（FMs）来举例说明。通过肉眼观察到的截断值分别为 1ng/mL 和 50ng/mL，实现了对生和加工食品中 AFB1 和 FMs 污染的可重现检测。根据与特定颜色代码相对应的标签颜色对污染物进行了识别。此外，通过使用普通智能手机获取条带图像，并通过 RGB 数据分析进行分析，从而对两种霉菌毒素进行半定量检测。
