一种用于同时检测脱氧雪腐镰刀菌烯醇、伏马毒素B和黄曲霉毒素B的侧向流动分析法。
A lateral flow assay for simultaneous detection of Deoxynivalenol, Fumonisin B and Aflatoxin B.
作者信息
Yu Songcheng, He Leiliang, Yu Fei, Liu Lie, Qu Chenling, Qu Lingbo, Liu Jie, Wu Yuming, Wu Yongjun
机构信息
College of Public Health, Zhengzhou University, Zhengzhou 450001, China.
College of Grain Oil and Food Science, Henan University of Technology, Zhengzhou 450052, China.
出版信息
Toxicon. 2018 Dec 15;156:23-27. doi: 10.1016/j.toxicon.2018.10.305. Epub 2018 Nov 3.
Deoxynivalenol (DON), Fumonisin B (FB) and Aflatoxin B (AFB) have strong toxicity to humans and exist widely in grain and food. It is necessary to screen these mycotoxins to guarantee food safety. In order to develop a rapid, simple, low-cost and simultaneous-detection method, composites of antibody-nano-Au-particles, DON-BSA, FB-BSA, and AFB-BSA were prepared to establish a lateral flow assay based on competitive inhibition. The results suggested that the visual detection limits for DON, FB and AFB were 10, 30, and 10 ng mL, respectively. On the other hand, it had no reactivity to T-2 toxin, zearalenone, ochratoxin A and nivalenol, which demonstrates good specificity. Besides, the strip had good repeatability and stability. Therefore, DON, FB and AFB would be screened simultaneously by lateral flow assay for food safety.
脱氧雪腐镰刀菌烯醇（DON）、伏马菌素B（FB）和黄曲霉毒素B（AFB）对人体具有很强的毒性，且广泛存在于谷物和食品中。为保证食品安全，有必要对这些霉菌毒素进行筛查。为开发一种快速、简便、低成本的同时检测方法，制备了抗体-纳米金颗粒、DON-BSA、FB-BSA和AFB-BSA复合物，以建立基于竞争抑制的侧向流动分析法。结果表明，DON、FB和AFB的目视检测限分别为10、30和10 ng/mL。另一方面，该方法对T-2毒素、玉米赤霉烯酮、赭曲霉毒素A和雪腐镰刀菌烯醇无反应，具有良好的特异性。此外，试纸条具有良好的重复性和稳定性。因此，可通过侧向流动分析法同时筛查食品中的DON、FB和AFB，以保障食品安全。
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