Franke Nora, Bette Michael, Marquardt André, Briese Thomas, Lipkin W Ian, Kurz Christopher, Ehrenreich Jovine, Mack Elisabeth, Baying Bianka, Beneš Vladimir, Rodepeter Fiona R, Neff Andreas, Teymoortash Afshin, Eivazi Behfar, Geisthoff Urban, Stuck Boris A, Bakowsky Udo, Mandic Robert
Department of Otolaryngology/Head and Neck Surgery, University Hospital Giessen and Marburg, Marburg, Germany.
Institute of Anatomy and Cell Biology, Philipps University, Marburg, Germany.
In Vivo. 2018 Nov-Dec;32(6):1323-1331. doi: 10.21873/invivo.11382.
BACKGROUND/AIM: Vascular anomalies encompass different vascular malformations [arteriovenous (AVM), lymphatic (LM), venous lymphatic (VLM), venous (VM)] and vascular tumors such as hemangiomas (HA). The pathogenesis of vascular anomalies is still poorly understood. Viral infection was speculated as a possible underlying cause.
A total of 13 human vascular anomalies and three human skin control tissues were used for viral analysis. RNA derived from AVM (n=4) and normal skin control (n=3) tissues was evaluated by RNA sequencing. The Virome Capture Sequencing Platform for Vertebrate Viruses (VirCapSeq-VERT) was deployed on 10 tissues with vascular anomalies (2×AVM, 1×HA, 1×LM, 2×VLM, 4×VM).
RNA sequencing did not show any correlation of AVM with viral infection. By deploying VirCapSeq-VERT, no consistent viral association was seen in the tested tissues.
The analysis does not point to the presence of an active viral infection in vascular anomalies. However, transient earlier viral infections, e.g. during pregnancy, cannot be excluded with this approach.
背景/目的：血管异常包括不同的血管畸形[动静脉畸形（AVM）、淋巴管畸形（LM）、静脉淋巴管畸形（VLM）、静脉畸形（VM）]以及血管瘤（HA）等血管肿瘤。血管异常的发病机制仍未完全清楚。病毒感染被推测为可能的潜在病因。
共使用13例人类血管异常组织和3例人类皮肤对照组织进行病毒分析。通过RNA测序评估来自动静脉畸形（n = 4）和正常皮肤对照（n = 3）组织的RNA。脊椎动物病毒宏基因组捕获测序平台（VirCapSeq-VERT）应用于10例血管异常组织（2例动静脉畸形、1例血管瘤、1例淋巴管畸形、2例静脉淋巴管畸形、4例静脉畸形）。
RNA测序未显示动静脉畸形与病毒感染有任何相关性。通过应用VirCapSeq-VERT，在测试组织中未发现一致的病毒关联。
该分析未表明血管异常中存在活跃的病毒感染。然而，这种方法不能排除早期短暂的病毒感染，例如孕期的病毒感染。
