Mikolajczyk Agata, Khosrawipour Veria, Schubert Justyna, Plociennik Michal, Nowak Kacper, Fahr Christian, Chaudhry Haris, Khosrawipour Tanja
Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland.
Department of Orthopedic and Trauma Surgery, Ortho Clinic, Dortmund, Dortmund, Germany.
In Vivo. 2018 Nov-Dec;32(6):1369-1372. doi: 10.21873/invivo.11388.
Pressurized aerosol chemotherapy (PAC) is a novel approach to the treatment of surface malignancies. This study aimed to investigate whether PAC is a feasible treatment of early-stage bladder cancer.
PAC via inserted microcatheter was performed on a fresh urinary bladder in a post-mortem swine model (n=3), creating a pressurized doxorubicin chemoaerosol. Drug penetration of aerosolized doxorubicin at different concentrations (3 mg/50 ml, 9 mg/50 ml and 15 mg/50 ml) and different locations on the mucosa was measured via fluorescence microscopy.
Mean endoluminal penetration rates for the urothelium following PAC reached 149±61 μm (using 15 mg/50 ml). Doxorubicin penetration was significantly increased with higher drug concentration (15 vs. 3 mg/50 ml: p<0.01). This study demonstrated the feasibility of PAC for intravesical use.
PAC is a feasible minimally-invasive approach to the treatment of early-stage bladder cancer.
加压气雾剂化疗（PAC）是一种治疗体表恶性肿瘤的新方法。本研究旨在探讨PAC是否是早期膀胱癌的一种可行治疗方法。
在死后猪模型（n = 3）的新鲜膀胱上通过插入微导管进行PAC，制备加压阿霉素化疗气雾剂。通过荧光显微镜测量不同浓度（3 mg/50 ml、9 mg/50 ml和15 mg/50 ml）的雾化阿霉素在黏膜不同位置的药物渗透情况。
PAC后尿路上皮的平均腔内渗透速率达到149±61μm（使用15 mg/50 ml时）。阿霉素的渗透随着药物浓度的升高而显著增加（15 mg/50 ml与3 mg/50 ml相比：p<0.01）。本研究证明了PAC膀胱内使用的可行性。
PAC是一种治疗早期膀胱癌的可行的微创方法。
