Iwahashi H, Negoro Y, Ikeda A, Kido R
J Chromatogr. 1987 Mar 27;391(1):199-205. doi: 10.1016/s0021-9673(01)94316-x.
Radical species were detected in the incubation mixtures of some retinoids (retinoic acid, retinal, retinol and retinyl acetate) by using the spin-trapping technique. The spin-adducts were resolved by high-performance liquid chromatography on a reversed-phase column with isocratic elution and detected by electron spin resonance spectroscopy and electrochemical detection. The spin-adducts were eluted in the order retinoic acid, retinol, retinyl acetate, in a similar manner to the retinoids themselves. These results suggest that the spin-adducts are products of nitrosobenzene with retinoid radicals in which the retinoid radicals retain their original chemical structures.
通过自旋捕集技术在一些类视黄醇（视黄酸、视黄醛、视黄醇和醋酸视黄酯）的孵育混合物中检测到了自由基物种。自旋加合物通过反相柱上的高效液相色谱法在等度洗脱条件下进行分离，并通过电子自旋共振光谱和电化学检测进行检测。自旋加合物按视黄酸、视黄醇、醋酸视黄酯的顺序洗脱，与类视黄醇本身的洗脱方式类似。这些结果表明，自旋加合物是亚硝基苯与类视黄醇自由基的产物，其中类视黄醇自由基保留了其原始化学结构。
