Kieber D J, Blough N V
Woods Hole Oceanographic Institution, Chemistry Department, Woods Hole, Massachusetts 02543.
Free Radic Res Commun. 1990;10(1-2):109-17. doi: 10.3109/10715769009145940.
A simple, highly sensitive method for the simultaneous determination of arrays of carbon-centered radicals in aqueous systems is described. Radicals are efficiently trapped by an amino-nitroxide to form stable products which are then reacted with fluorescamine to produce highly fluorescent adducts. The adducts are easily separated by reversed-phase high performance liquid chromatography. The detection limit for individual radical adducts (0.5 to 2 nM) is two to three orders of magnitude lower than those of current methods employing electron paramagnetic resonance detection. Results on the photolysis of ketones and alpha-keto acids demonstrate the potential of this technique. This approach should be widely applicable to the study of radical processes in biological and chemical systems.
本文描述了一种用于同时测定水性体系中碳中心自由基阵列的简单、高灵敏度方法。自由基通过氨基氮氧化物有效捕获形成稳定产物，然后该产物与荧光胺反应生成高荧光加合物。加合物通过反相高效液相色谱法轻松分离。单个自由基加合物的检测限（0.5至2 nM）比目前采用电子顺磁共振检测的方法低两到三个数量级。酮和α-酮酸光解的结果证明了该技术的潜力。这种方法应广泛适用于生物和化学体系中自由基过程的研究。
