Cecchi Francesca, Dadousis Christos, Bozzi Riccardo, Fratini Filippo, Russo Claudia, Bandecchi Patrizia, Cantile Carlo, Mazzei Maurizio
Department of Veterinary Sciences, University of Pisa, Viale delle Piagge 2, Pisa, Italy.
DISPAA-Sez. Scienze Animali, Università degli Studi di Firenze, Piazzale delle Cascine, 18, Firenze, Italy.
Trop Anim Health Prod. 2019 Mar;51(3):729-733. doi: 10.1007/s11250-018-1728-y. Epub 2018 Oct 22.
Small ruminant lentiviruses (SRLVs) are a heterogeneous group of viruses of sheep, goat, and wild ruminants responsible of lifelong persistent infection leading to a multisystem chronic disease. Increased evidences indicate that host genetic factors could influence the individual SRLV resistance. The present study was conducted on the Garfagnina goat breed, an Italian goat population registered on the Tuscan regional repertory of genetic resources at risk of extinction. Forty-eight adult goats belonging to a single flock were studied. SRLV diagnosis was achieved by serological tests and 21 serologically positive animals were identified. All animals were genotyped with the Illumina GoatSNP60 BeadChip and a genome-wide scan was then performed on the individual marker genotypes, in an attempt to identify genomic regions associated with the infection. One SNP was found significant (P < 5 × 10) on CHR 18 at 62,360,918 bp. The SNP was an intron of the zinc finger protein 331 (ZNF331) protein. In the region 1 Mb upstream the significant SNP, the NLRP12 (NLR family pyrin domain containing 12), the PRKCG (protein kinase C gamma), and the CACNG7 (calcium voltage-gated channel auxiliary subunit gamma 7) were found.
小反刍兽慢病毒（SRLVs）是绵羊、山羊和野生反刍动物中的一组异质性病毒，可导致终身持续性感染，引发多系统慢性疾病。越来越多的证据表明，宿主遗传因素可能影响个体对SRLV的抗性。本研究以加尔法尼亚纳山羊品种为对象，该品种是意大利的一个山羊种群，已被列入托斯卡纳地区濒危遗传资源名录。研究了来自单个羊群的48只成年山羊。通过血清学检测进行SRLV诊断，共鉴定出21只血清学阳性动物。所有动物均使用Illumina GoatSNP60 BeadChip进行基因分型，然后对个体标记基因型进行全基因组扫描，试图识别与感染相关的基因组区域。在18号染色体上62360918 bp处发现一个单核苷酸多态性（SNP）具有显著性（P < 5×10）。该SNP位于锌指蛋白331（ZNF331）蛋白的一个内含子中。在该显著SNP上游1 Mb区域，发现了NLRP12（含NLR家族pyrin结构域12）、PRKCG（蛋白激酶Cγ）和CACNG7（钙电压门控通道辅助亚基γ7）。
