Giammarioli M, Bazzucchi M, Puggioni G, Brajon G, Dei Giudici S, Taccori F, Feliziani F, De Mia G M
Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche, Via Salvemini 1, Perugia, Italy.
Virus Genes. 2011 Dec;43(3):380-4. doi: 10.1007/s11262-011-0653-1. Epub 2011 Aug 20.
In order to investigate the genetic heterogeneity of small ruminant lentivirus (SRLV) isolates in Italy, 55 clinical samples collected between 1998 and 2010 were analysed. The phylogenetic study was based on analysis of gag-pol sequences. Our findings revealed that the SRLVs belonged to the subtype A9 (n = 3, sheep), B1 (n = 5, goat), B2 (n = 3, sheep) and E2 (n = 5, goat). Interestingly, 39 isolates from both sheep and goat, significantly differed from all the other SRLVs previously described and formed two separate clusters within genotypes A and B tentatively named A11 (n = 27, goat and sheep) and B3 (n = 12, goat and sheep), which have never been shown before. These results revealed a marked diversity among Italian field SRLV strains which might reflect the absence of any systematic control measures.
为了研究意大利小反刍兽慢病毒（SRLV）分离株的遗传异质性，对1998年至2010年间收集的55份临床样本进行了分析。系统发育研究基于gag-pol序列分析。我们的研究结果显示，SRLV属于A9亚型（n = 3，绵羊）、B1亚型（n = 5，山羊）、B2亚型（n = 3，绵羊）和E2亚型（n = 5，山羊）。有趣的是，来自绵羊和山羊的39个分离株与先前描述的所有其他SRLV有显著差异，并在基因型A和B内形成了两个单独的簇，暂命名为A11（n = 27，山羊和绵羊）和B3（n = 12，山羊和绵羊），这是以前从未见过的。这些结果揭示了意大利野外SRLV毒株之间存在显著差异，这可能反映出缺乏任何系统的控制措施。
