用于研究慢性淋巴细胞白血病与单核细胞相互作用的体外试验

In Vitro Assay to Study CLL and Monocyte Interactions.

作者信息

Bertilaccio Maria Teresa Sabrina, Zhang Ronghua, Banerjee Priyanka, Gandhi Varsha

机构信息

Department of Experimental Therapeutics, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.

出版信息

Methods Mol Biol. 2019;1881:113-119. doi: 10.1007/978-1-4939-8876-1_9.

DOI:10.1007/978-1-4939-8876-1_9

PMID:30350201

Abstract

Investigations focusing on CLL and microenvironment interaction allow understanding role of each component of the microenvironment. In vitro cell depletion assay we described here enables us to evaluate the depletion of CLL cells and monocyte populations upon treatment with drugs targeting the interactions between CLL cells and monocytes. The assay is based on a quantitative multi-color flow cytometry analysis and, when combined to fluorescence-activated cell sorting and RT-PCR, it allows the isolation of CLL/monocyte cells and the further characterization of apoptotic and/or inflammatory pathways induced eventually on CLL cells and on monocytes.

摘要

聚焦于慢性淋巴细胞白血病（CLL）与微环境相互作用的研究有助于理解微环境各组分的作用。我们在此描述的体外细胞清除试验使我们能够评估在用靶向CLL细胞与单核细胞间相互作用的药物治疗后CLL细胞和单核细胞群体的清除情况。该试验基于定量多色流式细胞术分析，并且当与荧光激活细胞分选和逆转录聚合酶链反应相结合时，它能够分离CLL/单核细胞，并进一步表征最终在CLL细胞和单核细胞上诱导的凋亡和/或炎症途径。