ITMO University , St. Petersburg 191002 , Russian Federation.
Bioconjug Chem. 2018 Nov 21;29(11):3793-3799. doi: 10.1021/acs.bioconjchem.8b00648. Epub 2018 Nov 5.
The development of stimuli-responsive nanocontainers is an issue of utmost importance for many applications such as targeted drug delivery, regulation of the cell and tissue behavior, making bacteria have useful functions and here converting light. The present work shows a new contribution to the design of polyelectrolyte (PE) containers based on surface modified mesoporous titania particles with deposited Ag nanoparticles to achieve chemical light upconversion via biofilms. The PE shell allows slowing down the kinetics of a release of loaded l-arabinose and switching the bacteria luminescence in a certain time. The hybrid TiO/Ag/PE containers activated at 980 nm (IR) illumination demonstrate 10 times faster release of l-arabinose as opposed to non-activated containers. Fast IR-released l-arabinose switch bacteria fluorescence which we monitor at 510 nm. The approach described herein can be used in many applications where the target and delayed switching and light upconversion are required.
刺激响应型纳米容器的发展对于许多应用至关重要，例如靶向药物输送、调节细胞和组织行为、使细菌具有有用的功能以及在这里转换光。本工作展示了一种新的基于表面修饰的介孔二氧化钛颗粒的聚电解质（PE）容器的设计贡献，这些颗粒沉积有 Ag 纳米颗粒，以通过生物膜实现化学上的上转换发光。PE 壳允许减缓负载的 l-阿拉伯糖的释放动力学，并在特定时间切换细菌发光。在 980nm（IR）照射下激活的 TiO/Ag/PE 容器的 l-阿拉伯糖释放速度比非激活容器快 10 倍。快速的 IR 释放的 l-阿拉伯糖可以切换我们在 510nm 处监测到的细菌荧光。本文所描述的方法可用于许多需要目标和延迟切换以及上转换的应用中。
