Department of Zoology, Genetics and Physical Anthropology , University of Santiago de Compostela , Santiago de Compostela 15782 , Spain.
Meat Technology Center of Galicia, r/Galicia 4 , Parque Tecnolóxico de Galicia , San Cibrao das Viñas , Ourense 32900 , Spain.
J Agric Food Chem. 2018 Nov 7;66(44):11864-11872. doi: 10.1021/acs.jafc.8b03203. Epub 2018 Oct 24.
Patatin is the major tuber storage protein constituted by multiple isoforms highly variable across potato ( S. tuberosum) varieties. Here, we report a first association study of the variability of patatin isoforms between cultivars with their differences in tuber quality traits. Patatin-based proteomic distances were assessed between 15 table and/or processing potato cultivars from profiles of patatin obtained by two-dimensional electrophoresis. The content of ash, dry matter, reducing sugars, starch, total protein, and amino acid composition was also evaluated in tubers of each cultivar. Results showed that proteomic distances were significantly ( P < 0.05) associated with differences in the content of ash, dry matter, and essential amino acids. Proteomic distances were also able to identify outlier cultivars regarding the content of dry matter, content of protein, and protein quality. In conclusion, patatin-based proteomic distances can shorten the screening and selection processes of potato cultivars with advantageous characteristics in molecular breeding.
类脂素是主要的薯蓣贮藏蛋白，由多种在马铃薯（S. tuberosum）品种间高度变化的同工型组成。在这里，我们报告了对不同品种的类脂素同工型的多样性与其块茎品质特性差异之间的首次关联研究。通过二维电泳获得的类脂素图谱，在 15 个用于餐桌和/或加工的马铃薯品种之间评估了基于类脂素的蛋白质组学距离。还评估了每个品种的块茎中的灰分、干物质、还原糖、淀粉、总蛋白和氨基酸组成的含量。结果表明，蛋白质组学距离与灰分、干物质和必需氨基酸含量的差异显著相关（P < 0.05）。蛋白质组学距离还能够识别出关于干物质含量、蛋白质含量和蛋白质质量的异常品种。总之，基于类脂素的蛋白质组学距离可以缩短分子育种中具有有利特性的马铃薯品种的筛选和选择过程。
