Wolff L F, Pihlstrom B L, Bakdash M B, Schaffer E M, Jensen J R, Aeppli D M, Bandt C L
J Periodontol. 1987 May;58(5):301-7. doi: 10.1902/jop.1987.58.5.301.
This study was designed to investigate the effect of conventional oral hygiene (n = 116 subjects) versus a salt and peroxide oral hygiene regimen (n = 115 subjects) on subgingival microorganisms. Subgingival plaque for microscopic evaluation was obtained from eight index tooth sites in each of 231 adult subjects. Microbial forms were microscopically identified at baseline, 8, 16, and 24 months. For both oral hygiene groups, cocci were increased (P less than 0.05) and motile rods were decreased (P less than 0.05) at 8 months and returned to baseline by 16 months. Spirochetes were decreased (P less than 0.05) and remained low through 24 months in both oral hygiene groups. The frequency of agreement between clinical (bleeding) and microbial (greater than or equal to 15% spirochetes or motile rods or greater than or equal to 20% spirochetes + motile rods) criteria for instrumentation was 59.8%. It was also found that fewer total instrumentations for test subjects were observed when microbiological criteria were used as compared with clinical criteria. The greater number of instrumentations based on clinical criteria was highly significant (P less than or equal to 0.001). A significant change in microbial signs associated with peridontal disease may be obtained with either a conventional oral hygiene or a salt and peroxide oral hygiene home care regimen.
本研究旨在调查传统口腔卫生方法（116名受试者）与盐和过氧化物口腔卫生方案（115名受试者）对龈下微生物的影响。从231名成年受试者的每人均8个指数牙位获取龈下菌斑用于显微镜评估。在基线、8个月、16个月和24个月时对微生物形态进行显微镜鉴定。对于两个口腔卫生组，球菌在8个月时均增加（P<0.05），活动杆菌减少（P<0.05），并在16个月时恢复至基线水平。两个口腔卫生组的螺旋体均减少（P<0.05），并在24个月内一直维持在较低水平。临床（出血）和微生物学（螺旋体或活动杆菌≥15%或螺旋体+活动杆菌≥20%）器械操作标准之间的一致率为59.8%。还发现，与临床标准相比，采用微生物学标准时观察到的试验对象的器械操作总数更少。基于临床标准的器械操作数量更多，差异具有高度显著性（P≤0.001）。采用传统口腔卫生方法或盐和过氧化物口腔卫生家庭护理方案，均可使与牙周病相关的微生物体征发生显著变化。
