Kline A D, Wüthrich K
J Mol Biol. 1986 Dec 20;192(4):869-90. doi: 10.1016/0022-2836(86)90034-3.
The 1H nuclear magnetic resonance (n.m.r.) spectrum of the alpha-amylase inhibitor Tendamistat was completely assigned with the use of phase-sensitive homonuclear two-dimensional n.m.r. The assignments include the non-labile protons of the 74 amino acid residues as well as the labile protons which exchange sufficiently slowly to be observed in H2O solution. The proton chemical shifts are listed at 50 degrees C and pH 3.2, which coincides with the conditions used for the determination of the three-dimensional structure of Tendamistat.
利用相敏同核二维核磁共振技术，完全确定了α-淀粉酶抑制剂 Tendamistat 的 1H 核磁共振（n.m.r.）谱。这些归属包括 74 个氨基酸残基的非不稳定质子以及在 H2O 溶液中交换足够缓慢从而能够被观测到的不稳定质子。质子化学位移是在 50℃和 pH 3.2 条件下列出的，这与用于测定 Tendamistat 三维结构的条件一致。
