Olesnicky Eugenia C, Antonacci Simona, Popitsch Niko, Lybecker Meghan C, Titus M Brandon, Valadez Racquel, Derkach Paul G, Marean Amber, Miller Katherine, Mathai Samuel K, Killian Darrell J
Department of Biology, University of Colorado Colorado Springs, Colorado Springs, CO 80918, United States.
Department of Molecular Biology, Colorado College, Colorado Springs, CO 80903, United States.
Dev Biol. 2018 Dec 15;444(2):116-128. doi: 10.1016/j.ydbio.2018.09.022. Epub 2018 Oct 21.
RNA binding proteins (RBPs) mediate posttranscriptional gene regulatory events throughout development. During neurogenesis, many RBPs are required for proper dendrite morphogenesis within Drosophila sensory neurons. Despite their fundamental role in neuronal morphogenesis, little is known about the molecular mechanisms in which most RBPs participate during neurogenesis. In Drosophila, alan shepard (shep) encodes a highly conserved RBP that regulates dendrite morphogenesis in sensory neurons. Moreover, the C. elegans ortholog sup-26 has also been implicated in sensory neuron dendrite morphogenesis. Nonetheless, the molecular mechanism by which Shep/SUP-26 regulate dendrite development is not understood. Here we show that Shep interacts with the RBPs Trailer Hitch (Tral), Ypsilon schachtel (Yps), Belle (Bel), and Poly(A)-Binding Protein (PABP), to direct dendrite morphogenesis in Drosophila sensory neurons. Moreover, we identify a conserved set of Shep/SUP-26 target RNAs that include regulators of cell signaling, posttranscriptional gene regulators, and known regulators of dendrite development.
RNA结合蛋白（RBPs）在整个发育过程中介导转录后基因调控事件。在神经发生过程中，果蝇感觉神经元内正常的树突形态发生需要许多RBPs。尽管它们在神经元形态发生中具有重要作用，但对于大多数RBPs在神经发生过程中参与的分子机制却知之甚少。在果蝇中，艾伦·谢泼德（Shep）编码一种高度保守的RBP，它调节感觉神经元中的树突形态发生。此外，秀丽隐杆线虫的直系同源基因sup-26也与感觉神经元树突形态发生有关。然而，Shep/SUP-26调节树突发育的分子机制尚不清楚。在这里，我们表明Shep与RNA结合蛋白拖车挂钩（Tral）、伊普西隆沙赫特尔（Yps）、贝儿（Bel）和聚腺苷酸结合蛋白（PABP）相互作用，以指导果蝇感觉神经元中的树突形态发生。此外，我们鉴定出一组保守的Shep/SUP-26靶RNA，其中包括细胞信号调节因子、转录后基因调节因子和已知的树突发育调节因子。
