Spine Center, Department of Orthopedics, Changzheng Hospital, Second Military Medical University, 415th Feng Yang Road, Shanghai 200003, PR China.
Spine Center, Department of Orthopedics, Changzheng Hospital, Second Military Medical University, 415th Feng Yang Road, Shanghai 200003, PR China; Department of Soft Tissue Injury Surgery, 403 Clinical Department, 210th Hospital of PLA, 885th Chang Jiang Road, Dalian 116021, PR China.
Spine J. 2019 Apr;19(4):735-743. doi: 10.1016/j.spinee.2018.10.008. Epub 2018 Oct 20.
The presence of ossification of posterior longitudinal ligament (OPLL) can lead to symptomatic spinal cord compression and myelopathy. The surgical approach in patients with myelopathy is influenced by the presence of OPLL. Diagnose of OPLL currently requires computed tomography which incurs a large dose of radiation. Circulating disease-specific microRNAs (miRNAs) may serve as promising diagnostic markers with no radiation and easy accessibility for OPLL patients.
The purpose of this study is to evaluate the accuracy and significance of OPLL-specific microRNAs in discriminating OPLL from normal and intervertebral disc degenerated (IDD) patients by detecting the microRNAs' plasma level.
STUDY DESIGN/PATIENT SAMPLES: The level of microRNAs in OPLL patients' plasma or serum were detected and compared to that of normal and IDD patients to evaluate the accuracy and significance of diagnosing OPLL.
Taking advantage of the high through-put microRNA sequencing data, we selectively tested the ten most differentially regulated microRNAs in patients with: (1) radiologically diagnosed OPLL (n = 68), (2) radiologically diagnosed disc herniated patients with no evidence of OPLL (n = 45), (3) non-OPLL and nonmyelopathy patients (n = 53).The feasibility of the biomarkers in identifying OPLL was assessed through analysis of sensitivity, specificity, accuracy, negative predictive value, positive predictive value, and area under the curve (AUC) values.
Of the ten miRNAs validated, miR-10a-3p, miR-10a-5p, miR-563, miR-210-3p, and miR-218-3p showed significance between OPLL and non-OPLL blood samples. While miR-10a-5p, miR-563, and miR-210-3p showed high accuracy and significance in identifying OPLL from other groups individually, and an index that combines these miRNAs achieved the highest accuracy and AUC among these individual miRNAs.
Analysis of miR-10a-5p, miR-563, and miR-210-3p may be of important value in diagnosing OPLL. These markers maybe useful in a clinical setting in the early detection of OPLL patients by blood testing.
后纵韧带骨化症（OPLL）的存在可导致脊髓受压和脊髓病症状。脊髓病患者的手术方法受 OPLL 的影响。OPLL 的诊断目前需要 CT 检查，这会产生大量辐射。循环疾病特异性 microRNAs（miRNAs）可能成为有前途的诊断标志物，因为它们没有辐射，并且易于 OPLL 患者获取。
本研究旨在通过检测 miRNA 的血浆水平，评估 OPLL 特异性 microRNAs 区分 OPLL 与正常和椎间盘退行性变（IDD）患者的准确性和意义。
研究设计/患者样本：检测 OPLL 患者血浆或血清中的 microRNA 水平，并与正常和 IDD 患者进行比较，以评估诊断 OPLL 的准确性和意义。
利用高通量 microRNA 测序数据，我们选择性地测试了以下患者的十种差异最明显的 microRNAs：（1）放射学诊断的 OPLL（n = 68），（2）放射学诊断无 OPLL 椎间盘突出症患者（n = 45），（3）非 OPLL 且无脊髓病患者（n = 53）。通过分析敏感性、特异性、准确性、阴性预测值、阳性预测值和曲线下面积（AUC）值来评估生物标志物识别 OPLL 的可行性。
在验证的十种 miRNA 中，miR-10a-3p、miR-10a-5p、miR-563、miR-210-3p 和 miR-218-3p 在 OPLL 与非 OPLL 血液样本之间显示出显著性差异。miR-10a-5p、miR-563 和 miR-210-3p 分别在单独识别 OPLL 时具有较高的准确性和显著性，而组合这些 miRNA 的指数在这些单个 miRNA 中具有最高的准确性和 AUC。
分析 miR-10a-5p、miR-563 和 miR-210-3p 可能对诊断 OPLL 具有重要价值。这些标志物可能通过血液检测有助于 OPLL 患者的早期检测。
