Nagakura K, Hayakawa M, Hata M, Nakamura H
J Urol. 1987 Jun;137(6):1300-3. doi: 10.1016/s0022-5347(17)44487-9.
The present studies were carried out in 11 human renal cell carcinomas to determine the presence of a receptor specific for an active form of vitamin D, 1,25-dihydroxyvitamin D3. Saturation and Scatchard analyses of the cytosol receptor for 1,25-dihydroxyvitamin D3 showed that nine tumors had a detectable level of the receptor (two fmol/mg. protein). The equilibrium dissociation constant of these receptors ranged between 46 and 380 pM and the binding capacity also ranged between 3.5 and 12.7 femtomol/mg. protein. Sucrose density gradient analysis of the specific binders revealed that the tumors had a receptor protein appearing as a single 3.6S peak. Two tumors which had only a trace of the receptor were high grade solid tumors consisting mainly of spindle or pleomorphic cells. Nine tumors possessing 1,25-dihydroxyvitamin D3 receptor consisted of clear and/or granular cells. Thus, the absence of the receptor was only accompanied by low differentiated sarcomatoid tumors with poor prognosis. However, so far, the amount of the receptor in the receptor-positive tumor did not relate to the other clinical and pathological features of the patients.
本研究对11例人类肾细胞癌进行，以确定维生素D活性形式1,25 - 二羟维生素D3特异性受体的存在情况。对1,25 - 二羟维生素D3胞浆受体进行的饱和及Scatchard分析表明，9个肿瘤有可检测水平的受体（2飞摩尔/毫克蛋白质）。这些受体的平衡解离常数在46至380皮摩尔之间，结合能力也在3.5至12.7飞摩尔/毫克蛋白质之间。对特异性结合物的蔗糖密度梯度分析显示，肿瘤有一种受体蛋白，表现为单一的3.6S峰。仅有微量受体的两个肿瘤是主要由梭形或多形性细胞组成的高级别实体瘤。9个具有1,25 - 二羟维生素D3受体的肿瘤由透明和/或颗粒细胞组成。因此，受体缺失仅伴有预后不良的低分化肉瘤样肿瘤。然而，到目前为止，受体阳性肿瘤中的受体数量与患者的其他临床和病理特征无关。
