Department of Microbiology, Faculty of Medical Sciences, University of Sri Jayewardenepura, Nugegoda, Sri Lanka.
Department of Chemistry, Faculty of Applied Sciences, University of Sri Jayewardenepura, Nugegoda, Sri Lanka.
Med Princ Pract. 2019;28(1):28-35. doi: 10.1159/000494757. Epub 2018 Oct 23.
Pseudomonas aeruginosa and Staphylococcus aureus dual-species biofilm infections are notoriously difficult to manage. This study aimed at investigating the influence of four different culture media on the planktonic growth, adhesion, and biofilm formation of P. aeruginosa and S. aureus.
We monitored four different culture media including Nutrient Broth, Brain Heart Infusion (BHI) broth, Luria-Bertani broth, and RPMI 1640 medium on the planktonic growth, adhesion, and biofilm formation of P. aeruginosa (ATCC 27853) and S. aureus (ATCC 25923) using MTT assay and scanning electron microscopy (SEM).
The most robust growth of the mono- and dual-species cultures was noted in BHI broth. On the contrary, RPMI 1640 medium promoted maximal initial adhesion of both the mono- and dual-species, but BHI broth fostered the maximal biofilm growth. SEM images showed profuse extracellular polysaccharide production in biofilms, particularly in coculture, in BHI medium.
Our data demonstrate that BHI broth, relative to the other tested media, is the most conducive for in vitro evaluation of biofilm and planktonic growth kinetics of these two pathogens, both in mono- and coculture.
铜绿假单胞菌和金黄色葡萄球菌的双物种生物膜感染是众所周知的难以处理。本研究旨在研究四种不同的培养基对铜绿假单胞菌和金黄色葡萄球菌浮游生长、黏附和生物膜形成的影响。
我们使用 MTT 法和扫描电子显微镜（SEM）监测四种不同的培养基，包括营养肉汤、脑心浸液（BHI）肉汤、Luria-Bertani 肉汤和 RPMI 1640 培养基，对铜绿假单胞菌（ATCC 27853）和金黄色葡萄球菌（ATCC 25923）的浮游生长、黏附和生物膜形成的影响。
在 BHI 肉汤中，单种和双种培养物的生长最为旺盛。相反，RPMI 1640 培养基促进了两种单种和双种的初始最大黏附，但 BHI 肉汤促进了最大的生物膜生长。SEM 图像显示，在 BHI 培养基中，生物膜中产生了丰富的胞外多糖，特别是在共培养物中。
我们的数据表明，与其他测试的培养基相比，BHI 肉汤最有利于这两种病原体的浮游生长和生物膜动力学的体外评估，无论是在单培养物还是共培养物中。
