Ronkainen Aki, Khan Imran, Satokari Reetta
Human Microbiome Research Program, Faculty of Medicine, University of Helsinki, Helsinki 00290, Finland.
Microbiome Res Rep. 2024 Nov 1;4(1):5. doi: 10.20517/mrr.2024.43. eCollection 2025.
To study the ability of bifidobacterial strains isolated from fecal donors to prevent pathogens from adhering to intestinal mucus, along with their antimicrobial susceptibility. Pathogen prevention was assessed through an adhesion assay using immobilized porcine mucus. Subsequently, bifidobacterial RNA-Seq data were analyzed to pinpoint glycoside hydrolases and glycosyltransferases possibly involved in mucus degradation affecting pathogen adhesion. The antimicrobial susceptibility of bifidobacterial strains was evaluated using susceptibility testing, followed by analysis of whole-genome sequencing data to reveal antimicrobial resistance genes. Bifidobacterial strains inhibited pathogen adhesion to intestinal mucus, with most strains reducing the adhesion levels of pathogens like , , Typhimurium, and by at least 70%. None of the strains significantly affected , but they moderately reduced the adhesion of . Gene expression analysis indicated that the more effective strains expressed higher levels of glycoside hydrolases, correlating with their pathogen exclusion capabilities. Antimicrobial susceptibility testing revealed that most strains were sensitive to several antibiotics, though some exhibited resistance to tobramycin, trimethoprim, and ciprofloxacin. Notably, one strain carried the gene, conferring resistance to tetracycline. The bifidobacterial strains characterized in this study show potential for bacteriotherapeutic applications due to their strong ability to interfere with the adhesion of pathogenic bacteria and their lack of alarming antimicrobial resistance patterns.
研究从粪便供体中分离出的双歧杆菌菌株阻止病原体粘附于肠道粘液的能力及其抗菌药敏性。通过使用固定化猪粘液的粘附试验评估病原体预防能力。随后，分析双歧杆菌RNA测序数据，以找出可能参与影响病原体粘附的粘液降解的糖苷水解酶和糖基转移酶。使用药敏试验评估双歧杆菌菌株的抗菌药敏性，随后分析全基因组测序数据以揭示抗菌抗性基因。双歧杆菌菌株抑制病原体对肠道粘液的粘附，大多数菌株将诸如、、鼠伤寒沙门氏菌和等病原体的粘附水平降低至少70%。没有菌株对有显著影响，但它们适度降低了的粘附。基因表达分析表明，更有效的菌株表达更高水平的糖苷水解酶，这与其病原体排除能力相关。抗菌药敏试验表明，大多数菌株对几种抗生素敏感，尽管有些菌株对妥布霉素、甲氧苄啶和环丙沙星表现出抗性。值得注意的是，一株菌株携带基因，赋予其对四环素的抗性。本研究中表征的双歧杆菌菌株因其强大的干扰病原菌粘附能力和缺乏令人担忧的抗菌抗性模式而显示出细菌治疗应用的潜力。
