Valadão Marisa C, Silva Beatriz C M, López-Hernández Danimar, Araújo Jackson V, Locke Sean A, Pinto Hudson A
Laboratório de Biologia de Trematoda, Departament of Parasitology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, 486, Belo Horizonte, Minas Gerais, 30123-970, Brazil.
Department of Veterinary, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil.
Parasitol Res. 2018 Dec;117(12):3927-3934. doi: 10.1007/s00436-018-6102-5. Epub 2018 Oct 23.
Postharmostomum commutatum (Dietz, 1858), a parasite of the caeca of poultry, has been reported from many different parts of the world. Despite its importance, there are no molecular sequences available and its phylogenetic position is unknown in relation to other members of Brachylaimoidea, a group in which taxonomic confusion reigns. Here, morphological and molecular techniques were used to study digeneans from the caeca of free-range chickens found naturally infected in the municipality of Viçosa, state of Minas Gerais, Brazil, between August 2017 and May 2018. The specimens were identified as P. commutatum, with Postharmostomum gallinum Witenberg, 1923 herein considered a junior synonym. Sequences obtained for the 28S, ITS2, and cox-1 genes were compared with sequences available from other species of Brachylaimoidea. Phylogenetic analysis of the three markers indicates P. commutatum formed an isolated lineage from other brachylaimoids, supporting the distinct status of the genus. The topology of phylogenetic trees obtained suggests that the morphology-based classification of families of Brachylaimoidea is artificial and new rearrangements of some genera or creation of new families may be necessary. The sequences newly obtained here will be useful for testing the cosmopolitan distribution of P. commutatum.
交合后殖吸虫（Postharmostomum commutatum）（迪茨，1858年）是家禽盲肠的一种寄生虫，已在世界许多不同地区被报道。尽管其具有重要性，但目前尚无分子序列，并且相对于短腺科（Brachylaimoidea）的其他成员，其系统发育位置尚不清楚，该类群存在分类学混乱的情况。在这里，我们运用形态学和分子技术，对2017年8月至2018年5月间在巴西米纳斯吉拉斯州维索萨市自然感染的散养鸡盲肠中的复殖吸虫进行了研究。这些标本被鉴定为交合后殖吸虫，本文将鸡后殖吸虫（Postharmostomum gallinum）维滕贝格，1923年视为次异名。将获得的28S、ITS2和cox - 1基因序列与短腺科其他物种的可用序列进行了比较。对这三个标记的系统发育分析表明，交合后殖吸虫与其他短腺科吸虫形成了一个孤立的谱系，支持了该属的独特地位。所得系统发育树的拓扑结构表明，基于形态学的短腺科分类是人为的，可能需要对一些属进行新的重新排列或创建新的科。这里新获得的序列将有助于检验交合后殖吸虫的全球分布情况。
