用脑膜炎奈瑟菌IgA1蛋白酶片段免疫的小鼠中抗脑膜炎球菌免疫形成的特点
Peculiarities of the Formation of Antimeningococcus Immunity in Mice Immunized with Fragments of N. meningitidis IgA1 Protease.
作者信息
Kotel'nikova O V, Alliluev A P, Zinchenko A A, Prokopenko Yu A, Zhigis L S, Zueva V S, Razgulyaeva O A, Gordeeva E A, Melikhova T D, Nokel' E A, Rumsh L D
机构信息
M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
出版信息
Bull Exp Biol Med. 2018 Oct;165(6):763-766. doi: 10.1007/s10517-018-4260-1. Epub 2018 Oct 23.
We studied immunogenicity of two recombinant proteins FR.9 and FR.11-3 created on the basis of fragments of the primary structure of N. meningitidis IgA1 protease with different molecular weights containing different sets of T and B epitopes. The proteins actively protect animals infected with live virulent culture of meningococci, serogroups A, B, and C. Analysis of CD4, CD8, and CD19 lymphocyte populations in mouse blood showed predominant contribution of different cell populations to the formation of immune response to different proteins. Injection of FR.11-3 protein to animals did no affect the immunoregulatory index, hence, this protein can be used for creation of immunologically safe vaccine preparation.
我们研究了基于脑膜炎奈瑟菌IgA1蛋白酶一级结构片段构建的两种重组蛋白FR.9和FR.11 - 3的免疫原性，这两种蛋白分子量不同，含有不同的T和B表位组合。这些蛋白能有效保护感染A、B和C血清群脑膜炎球菌活毒培养物的动物。对小鼠血液中CD4、CD8和CD19淋巴细胞群体的分析表明，不同细胞群体对不同蛋白免疫反应的形成有主要贡献。给动物注射FR.11 - 3蛋白不影响免疫调节指数，因此，该蛋白可用于制备免疫安全的疫苗制剂。
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