Morelli G, del Valle J, Lammel C J, Pohlner J, Müller K, Blake M, Brooks G F, Meyer T F, Koumaré B, Brieske N
Max-Planck Institut für molekulare Genetik, Berlin, Germany.
Mol Microbiol. 1994 Jan;11(1):175-87. doi: 10.1111/j.1365-2958.1994.tb00299.x.
Five murine epitopes were defined and mapped within IgA1 protease produced by Neisseria meningitidis. Epitopes 1 and 2 were present in IgA1 protease from all strains, and from Neisseria gonorrhoeae. Epitopes 3 through to 5 varied between subgroups of serogroup A meningococci, but have remained constant over decades within the subgroups, except for epitope 4, which changed between 1983 and 1987 during the spread of subgroup III meningococci from Asia to Africa. Binding of monoclonal antibodies to epitopes 1, 4 and 5 neutralized enzymatic function. Human sera containing antibodies to IgA1 protease as a result of natural infection inhibited binding of monoclonal antibodies to epitope 4 but not to the other epitopes.
在脑膜炎奈瑟菌产生的IgA1蛋白酶中确定并定位了五个鼠源表位。表位1和表位2存在于所有菌株以及淋病奈瑟菌的IgA1蛋白酶中。表位3至表位5在A群脑膜炎球菌亚组之间存在差异，但在各亚组内数十年来保持不变，不过表位4在1983年至1987年期间，随着III型脑膜炎球菌从亚洲传播到非洲而发生了变化。单克隆抗体与表位1、4和5的结合中和了酶活性。因自然感染而含有针对IgA1蛋白酶抗体的人血清抑制了单克隆抗体与表位4的结合，但不抑制与其他表位的结合。
