Fedorova T N, Devyatov A A, Berezhnoi D S, Stvolinskii S L, Morozova M P, Gavrilova S A, Tutelyan V A
Research Center of Neurology, Moscow, Russia.
Federal Research Center of Nutrition and Biotechnology, Moscow, Russia.
Bull Exp Biol Med. 2018 Oct;165(6):746-750. doi: 10.1007/s10517-018-4256-x. Epub 2018 Oct 23.
Oxidative status was assessed in different areas of the cerebral cortex of male Wistar rats under normal condition and during permanent 24-h focal ischemia. In intact animals, the level of lipid hydroperoxides in the frontal lobes of both hemispheres was by 36% higher than in other cortical areas, while total antioxidant activity was by 25% higher than in other areas. During ischemia, changes in oxidative status were localized only in the ischemic focus and penumbra zone and did not involve other cortical areas. We demonstrated for the first time a neuroprotective effect of therapeutic administration of carnosine in low doses (50 mg/kg) on parameters of the oxidative status under conditions of focal ischemia comparable to its effect of high doses (500 mg/kg) as well as its local effect in the penumbra zone. A dose-dependent effect of carnosine on antioxidant activity in the penumbra zone during ischemia was also demonstrated. These findings confirm effectiveness of not only preventive carnosine administration, but also its application in the postischemic period of the stroke.
在正常条件下以及在持续24小时局灶性缺血期间，对雄性Wistar大鼠大脑皮质的不同区域进行氧化状态评估。在完整动物中，双侧额叶中脂质氢过氧化物水平比其他皮质区域高36%，而总抗氧化活性比其他区域高25%。在缺血期间，氧化状态的变化仅局限于缺血灶和半暗带，未涉及其他皮质区域。我们首次证明，低剂量（50mg/kg）肌肽治疗给药对局灶性缺血条件下氧化状态参数具有神经保护作用，其效果与高剂量（500mg/kg）相当，并且在半暗带具有局部作用。还证明了缺血期间肌肽对半暗带抗氧化活性的剂量依赖性作用。这些发现证实了肌肽不仅预防性给药有效，而且在中风缺血后时期应用也有效。
